Human p53 ELISA Kit (Colorimetric)

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Human p53 ELISA Kit [NBP1-91257] - Data for Human p53 Platinum ELISA

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Cell culture supernatant, serum
Standard Curve Range
0.78 - 50 U/mL
Sensitivity
0.33 U/mL

Order Details

Human p53 ELISA Kit (Colorimetric) Summary

Standard Curve Range
0.78 - 50 U/mL
Sensitivity
0.33 U/mL
Assay Type
Sandwich ELISA
Inter-Assay
8.90%
Intra-Assay
5.50%
Spike Recovery
95%
Sample Volume
50 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
TP53

Applications/Dilutions

Application Notes
The human p53 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of human p53.

Reactivity Notes

Human

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
  2. Streptavidin-HRP
  3. Substrate Solution (tetramethyl-benzidine)
  4. Sample Diluent
  5. Stop Solution (1M Phosphoric acid)
  6. Biotin-Conjugate anti-human p53 monoclonal antibody
  7. Human p53 Standard lyophilized, 100 U/ml upon reconstitution
  8. Adhesive Films
  9. Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to human p53
  10. Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)

Alternate Names for Human p53 ELISA Kit (Colorimetric)

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Background

p53 is a tumor suppressor that is regulated by transcriptional, post-transcriptional, and post-translational mechanisms in response to stress. Studies demonstrate that p53 is critical for the prevention of tumorigenesis and may potentially be involved in at least half of all known cancers. p53 functions as a transcription factor to regulate genes involved in apoptosis, cell cycle, senescence, metabolism, angiogenesis, immunity, differentiation, and migration. Moreover, p53 is phosphorylated at multiple sites, including Ser15, following DNA damage.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Blogs on p53. Showing 1-10 of 16 blog posts - Show all blog posts.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

PRMT6: One Function, Many Roles
Protein arginine methylation is a prevalent posttranslational modification in eukaryotic cells. It regulates RNA processing, trafficking and nascent pre-RNA metabolism, receptor-mediated signal transduction, and transcriptional activation processes. P...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

p14 ARF is an Important Tumor Suppressor
The p14ARF (Alternative Reading Frame) tumor suppressor is a protein product of the alternative reading frame (ARF) of the human INK4a locus which regulates a series of cell cycle regulatory proteins to promote cell cycle arrest in response to abnorma...  Read full blog post.

Showing 1-10 of 16 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol TP53
Entrez