p53 Antibody (SPM589) [Alexa Fluor® 488]

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Product Details

Summary
Reactivity Hu, Mu(-), Rt(-)Species Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P
Clone
SPM589
Clonality
Monoclonal
Host
Mouse
Conjugate
Alexa Fluor 488

Order Details

p53 Antibody (SPM589) [Alexa Fluor® 488] Summary

Immunogen
Recombinant human wild-type p53 protein
Marker
Nuclear
Specificity
Recognizes a 53kDa protein, which is identified as p53 suppressor gene product. It reacts with the mutant as well as the wild form of p53 under denaturing and non-denaturing conditions. Its epitope maps within the N-terminus (aa 20-25) of p53 oncoprotein. p53 is a tumor suppressor gene expressed in a wide variety of tissue types and is involved in regulating cell growth, replication, and apoptosis. It binds to MDM2, SV40 T antigen and human papilloma virus E6 protein. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia. Mutations involving p53 are found in a wide variety of malignant tumors, including breast, ovarian, bladder, colon, lung, and melanoma.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
TP53
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Reactivity Notes

Does not react with Mouse or Rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A or G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for p53 Antibody (SPM589) [Alexa Fluor® 488]

  • Antigen NY-CO-13
  • BCC7
  • FLJ92943
  • LFS1
  • LFS1TRP53
  • p53 tumor suppressor
  • p53
  • P53cellular tumor antigen p53
  • Phosphoprotein p53
  • TP53
  • transformation-related protein 53
  • TRP53
  • tumor protein p53
  • Tumor suppressor p53

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34817AF350
Alexa Fluor 405 NBP2-34817AF405
Alexa Fluor 488 NBP2-34817AF488
Alexa Fluor 532 NBP2-34817AF532
Alexa Fluor 594 NBP2-34817AF594
Alexa Fluor 647 NBP2-34817AF647
Alexa Fluor 700 NBP2-34817AF700
Alexa Fluor 750 NBP2-34817AF750
Allophycocyanin NBP2-34817APC
Biotin NBP2-34817B
DyLight 350 NBP2-34817UV
DyLight 405 NBP2-34817V
DyLight 488 NBP2-34817G
DyLight 550 NBP2-34817R
DyLight 594 NBP2-34817DL594
DyLight 650 NBP2-34817C
DyLight 680 NBP2-34817FR
DyLight 755 NBP2-34817IR
FITC NBP2-34817F
HRP NBP2-34817H
Janelia Fluor 549 NBP2-34817JF549
Janelia Fluor 646 NBP2-34817JF646
PE NBP2-34817PE
PerCP NBP2-34817PCP

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Bioinformatics Tool for p53 Antibody (NBP2-34817AF488)

Discover related pathways, diseases and genes to p53 Antibody (NBP2-34817AF488). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on p53. Showing 1-10 of 19 blog posts - Show all blog posts.

Developmental regulator Daam2 promotes glial cell tumors by degrading Von Hippel-Lindau protein
By Jamshed Arslan Pharm.D. Glioblastoma is an aggressive type of cancer that forms from the star-shaped glial cells of the central nervous system, called astrocytes. Intriguingly, several genes linked to glioblasto...  Read full blog post.

Autophagy independent roles of the core ATG proteins
By Christina Towers, PhD. Autophagy and ATG ProteinsAutophagy is a nutrient recycling process that cells use to fuel metabolism, particularly in response to nutrient deprivation.  It is critical for removal of dam...  Read full blog post.

Killing two birds with one stone: Treating inflammation and cancer by inhibiting prolyl-4-hydroxylase-1
By Jamshed Arslan Pharm.D. The cell’s oxygen-sensing machinery comprises prolyl-4-hydroxylases (P4Hs 1-3, PHDs 1-3, or EGLN 1-3) and their canonical target hypoxia-inducible factors (HIFs). When oxygen levels ...  Read full blog post.

Pathway Highlight: Which caspase substrates contribute to the morphological features associated with apoptosis?
Apoptosis, or programmed cell death, is controlled by a caspase signal cascade that activates downstream signals to induce the morphological changes used to differentiate apoptosis from other forms of cell death.  Novus Biologicals offers a variet...  Read full blog post.

The role of p53 in UV radiation DNA damage and subsequent tumorogenesis
p53, the protein product of the tp53 gene, is one of the most widely studied tumor suppressor proteins in cancer research.  p53 is unique in that it demonstrates both tumor suppressive and tumor progressive properties depending on whether it is fu...  Read full blog post.

MAPK8/JNK1 - A multifunctional kinase and drug target for cancer therapeutics
The c-Jun N-terminal kinase (JNK) family is a group of regulatory kinases with important functions in cell morphogenesis, inflammation, differentiation, and cell death (1). Aberrant activation of JNK family proteins in cancers has led to interest i...  Read full blog post.

p53 - Investigating an important tumor suppressor
p53 is a tumor suppressor that has a central role in regulating cell cycle arrest, DNA repair, and apoptosis. p53 is widely studied for its role in cancer and is mutated or altered in more than half of all cancers (1). This widespread role in tumor...  Read full blog post.

ATM - detecting and responding to DNA damage
Ataxia telangiectasia mutated (ATM) is essential for the maintenance of genomic stability. ATM is a 370 kDa serine-threonine kinase that is constitutively expressed in various tissues. Although primarily nuclear, ATM is also found at lower levels ...  Read full blog post.

NOXA - a BH3-only protein balancing cell death decisions
Noxa is a BH3-only protein involved in regulating cell death decisions. Noxa is a primary p53-response gene and is upregulated in response to p53 overexpression or DNA damage. Noxa can also be induced by alternative mechanisms including through a ...  Read full blog post.

p73: An Important Tumor Suppressor Cousin of p53
p73 has been identified as a long-lost cousin of the p53 tumor suppressor protein. It has high homology with both p53 and with p63, a gene implicated in the maintenance of epithelial stem cells. The presence of significant homology between the DNA-bin...  Read full blog post.

Showing 1-10 of 19 blog posts - Show all blog posts.

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Bioinformatics

Gene Symbol TP53