p53 [p Ser392] Antibody (FP3.2 [FPS392]) - BSA Free

Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - Wild-type p53 expressed in human trophoblast (paraffin-embedded sections). 1A - anti-p53 (total).

Immunohistochemistry: p53 [p Ser392] Antibody (FP3.2 [FPS392]) [NB500-575] - 1B anti-P53 (phospho Ser392) Note that some of total p53 positive nuclei are also FP3.2 (phospho p53) positive.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: FP3.2 [FPS392]
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

p53 [p Ser392] Antibody (FP3.2 [FPS392]) - BSA Free Summary

• Description: Novus Biologicals Mouse p53 [p Ser392] Antibody (FP3.2 [FPS392]) - BSA Free (NB500-575) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: KLH-conjugated phosphopeptide RHKKLMFKTEGPDS[P]D, corresponding to amino acids 378-393 of human p53.
• Modification: p Ser392
• Specificity: The antibody FP3.2 [FPS392] reacts with human p53 tumour suppressor protein phosphorylated at CKII site (Ser 392).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TP53
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Western Blot 1 ug/ml
• Application Notes: Immunohistochemistry-Paraffin sections - Staining technique: standard ABC technique (DAB+) Pretreatment: high temperature antigen retrieval (microwave, pressure cooker) in 10 mM citrate buffer pH 6.0 or 1 mM EDTA-NaOH buffer pH 8.0 Recommended dilution: 10 ug/ml Incubation: 1 hour at RT; or overnight at 4C
• Control:

  p53 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Phosphate buffered saline (PBS), pH 7.4
• Preservative: 15mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for p53 [p Ser392] Antibody (FP3.2 [FPS392]) - BSA Free

• Antigen NY-CO-13
• BCC7
• FLJ92943
• LFS1
• LFS1TRP53
• p53 tumor suppressor
• p53
• P53cellular tumor antigen p53
• Phosphoprotein p53
• TP53
• transformation-related protein 53
• TRP53
• tumor protein p53
• Tumor suppressor p53

Background

The tumour suppressor protein p53 is a key element of intracellular anticancer protection. It mediates cell cycle arrest or apoptosis in response to DNA damage or to starvation for pyrimidine nukleotides. It is up-regulated in response to these stress signals and stimulated to activate transcription of specific genes, resulting in expression of p21waf1 and other proteins involved in G1 or G2/M arrest, or proteins that trigger apoptosis, such as Bcl-2. The structure of p53 comprises N-terminal transactivation domain, central DNA-binding domain, oligomerisation domain, and C-terminal regulatory domain. There are various phosphorylation sites on p53, of which the phosphorylation at Ser15 is important for p53 activation and stabilization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TP53
• Entrez:
  • 7157(Human)
  • 22059(Mouse)
• Uniprot:
  • P04637()