p38 alpha/beta/gamma Antibody (PSH03-60)

Western Blot: p38 alpha/beta/gamma Antibody (PSH03-60) [NBP3-33147] - Western blot analysis of p38 alpha/beta/gamma on different lysates with Rabbit anti-p38 alpha/beta/gamma antibody (NBP3-33147) at 1/2,000 dilution. Lane 1: 293T cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: MDA-MB-231 cell lysate Lane 5: COS-1 cell lysate Lane 6: Neuro-2a cell lysate Lane 7: PC-12 cell lysate Lane 8: Mouse skeletal muscle tissue lysate Lane 9: Rat skeletal muscle tissue lysate Lane 10: Rat heart tissue lysate Lysates/proteins at 30 ug/Lane. Predicted band size: 42 kDa Observed band size: 42 kDa Exposure time: 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-33147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4C overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1/50,000 dilution was used for 1 hour at room temperature.

• Reactivity: Hu, Mu, Rt, Pm
• Applications: WB
• Clone: PSH03-60
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

p38 alpha/beta/gamma Antibody (PSH03-60) Summary

• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant protein within human MAPK12 aa 1-367 / 367. (Uniprot: Q16539)
• Localization: Cytoplasm, Nucleus, Mitochondrion.
• Specificity: Anti-p38 alpha/MAPK14 + p38 beta/MAPK11 + p38 gamma/MAPK12 Antibody [PSH03-60] detects endogenous levels of total p38 alpha, p38 beta and p38 gamma MAPK protein. This antibody does not recognize p38 delta MAPK protein.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MAPK14
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:2000
• Theoretical MW: 42 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.1% BSA and 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for p38 alpha/beta/gamma Antibody (PSH03-60)

• MAPK11
• MAPK12
• MAPK14
• p38
• p38ALPHA
• p38Beta
• p38gamma

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK14