p38 alpha [p Tyr323] Antibody

Western Blot: p38 alpha [p Tyr323] Antibody [NBP3-23249] - A) Western blot image of GST-recombinant p38 (K53M) mutant kinase untreated (lanes 1 & 3) or treated with Fyn kinase (lanes 2 & 4). B) Western blot analysis of p38 phosphorylation in mouse macrophages stimulated with 1 mM pervanadate for 30 min. (lanes 1 & 3) then the blot was treated with alkaline phosphatase (lanes 2 & 4). Both blots were probed with anti-p38 alpha (a.a. 319-328) (lanes 1 & 2) or anti-p38 alpha (Tyr-323) (lanes 3 & 4).

Immunocytochemistry/Immunofluorescence: p38 alpha [p Tyr323] Antibody [NBP3-23249] - Immunocytochemical labeling of p38 MAPK in pervanadate-treated mouse C2C12. The cells were labeled with mouse monoclonal p38 alpha MAPK and rabbit polyclonal p38 MAPK (Tyr-323) antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

• Reactivity: Hu, Mu, Rt, Ch
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p38 alpha [p Tyr323] Antibody Summary

• Description: Stable for at least 1 year at -20°C.
• Immunogen: Phospho-p38a MAP Kinase (Tyr-323) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding tyrosine 323 in mouse p38a. This peptide sequence is highly conserved in human and rat p38a, and has high homology to the conserved site in p38 beta. [UniProt# P47811]
• Modification: p Tyr323
• Specificity: This antibody was cross-adsorbed to a phospho-tyrosine peptide before affinity purification using phospho-p38 alpha (Tyr-323) peptide (without carrier). The antibody detects a 38 kDa band corresponding to p38a on SDS-PAGE immunoblots of human Jurkat, K562, and mouse macrophage (J774A.1) cells treated with pervanadate. In addition, the antibody detects recombinant p38 MAP Kinase when treated with Fyn kinase.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAPK14
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:300
  • Western Blot 1:1000
• Theoretical MW: 38 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: PBS, 1 mg/ml BSA and 50% glycerol
• Preservative: 0.05% Sodium Azide
• Purity: Antigen Affinity-purified

Alternate Names for p38 alpha [p Tyr323] Antibody

• CSBP;CSBP1;CSBP2;CSPB1;EXIP;MAPK14;Mitogen-activated protein kinase;Mitogen-activated protein kinase 14;Mxi2;p38;p38ALPHA;PRKM14;PRKM15;RK;SAPK2A
• MAPK14
• p38 alpha
• SAPK2a

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MAPK14