p38 alpha [p Tyr182] Antibody

Images

 
Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Human breast cancer cell line MDA-MB-231 was treated with different doses of p38 inhibitor and the expression of phospho-p38 and total p38 were detected by ...read more
Immunohistochemistry: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of paraffin-embedded human breast carcinoma tissue, using P38 MAPK (phospho- Tyr182) antibody.
Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182) antibody.
Western Blot: p38 alpha [p Tyr182] Antibody [NB100-82097] - Analysis of extracts from Hela cells, treated with TNFa or calf intestinal phosphatase (CIP).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

p38 alpha [p Tyr182] Antibody Summary

Immunogen
The antiserum was produced against synthesized phosphopeptide derived from human p38 alpha around the phosphorylation site of tyrosine 182 (T-G-YP-V-A).
Modification
p Tyr182
Specificity
Detects endogenous levels of p38 alpha only when phosphorylated at tyrosine 182.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPK14
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:50-1:100
  • Immunohistochemistry-Paraffin 1:50-1:100
  • Western Blot 1:500-1:1000
Theoretical MW
43 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
p38 alpha Overexpression Lysate
Reviewed Applications
Read 1 Review rated 4
using
NB100-82097 in the following applications:

Publications
Read Publication using
NB100-82097 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for p38 alpha [p Tyr182] Antibody

  • MAPK14
  • p38 alpha
  • SAPK2a

Background

p38 is a 38 kDa Stress Activated Protein Kinase / Map Kinase (SAPK / MAPK) that is fully activated by dual phosphorylation on threonine 180 and tyrosine 182, within the activation loop. p38 MAPK plays a critical role in the initiation of G2 delay after ultraviolet radiation; gene knockout studies have also revealed a critical role for p38 in cardiac remodeling. Downstream targets of p38 include the transcription factors ELK1 and ATF2 and the kinases MAPKAPK2 and MAPKAPK5. p38 MAPK plays a role in the production of IL6 and is thought to stabilize erythropoietin production during hypoxic stress.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for p38 Antibody (NB100-82097)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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Review for p38 Antibody (NB100-82097) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB100-82097:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot p38 alpha NB100-82097
Enlarge
4
reviewed by:
Haiquan Lu
WB Human 10/24/2018
View

Summary

ApplicationWestern Blot
Sample TestedHuman breast cancer cell lines
SpeciesHuman
Lot9514

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Control Lysate(s)

Secondary Antibodies

 

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Haiquan Lu
10/24/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol MAPK14
Entrez
Uniprot