P-Selectin/CD62P Antibody

Western Blot: P-Selectin/CD62P Antibody [NBP1-50764] - Analysis of P-Selectin expression in Jurkat cell lysate.

Product Discontinued

P-Selectin/CD62P Antibody Summary

• Description: This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab59738)
• Immunogen: Synthetic peptide surrounding amino acid 756 of mouse CD62P
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: SELP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 0.5 - 4 ug/mL
• Theoretical MW: 91 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2), 0.5% BSA, 30% Glycerol
• Preservative: 0.01% Thimerosal
• Concentration: 0.5 mg/ml
• Purity: Affinity purified

Alternate Names for P-Selectin/CD62P Antibody

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

CD62P is a member of the small selectin family of cellular adhesion molecules, which also includes CD62E and CD62L. Its structure, similar to the other members of the selectin family, consists of an N terminal lectin like domain of C type, followed by an epidermal growth factor like motif, a series of short consensus repeats, a transmembrane domain, and a cytoplasmic tail. The CD62P antigen is a 140 kDa glycoprotein, located in the alpha granules and the dense granules of platelets and endothelial cells. Activation of these cells results in rapid mobilization of CD62P from the storage granules to the cell surface. Activated platelets have a stable CD62P expression, while the endothelial cells lose CD62P expression within 1 h of activation, because of endocytosis of the molecule. CD62P is also expressed on megakaryocytes, but resting platelets and endothelial cells show no surface staining of CD62P.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for P-Selectin/CD62P Antibody (NBP1-50764)(1)

Publication using NBP1-50764

• Hu S, Wang X, Li Z et al. Platelet membrane and stem cell exosome hybrids enhance cellular uptake and targeting to heart injury Nano Today 2021-08-01 [PMID: 34306170]

Bioinformatics

• Gene Symbol: SELP
• Entrez:
  • 6403(Human)
  • 20344(Mouse)
  • 25651(Rat)
• Uniprot:
  • Q01102()