P-Selectin/CD62P Antibody (9E1) [Fluorescein]

Whole blood monocytes cells were stained with Mouse Anti-Human P‑Selectin/CD62P Fluorescein‑conjugated Monoclonal Antibody (Catalog # BBA34, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 9E1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Fluorescein

P-Selectin/CD62P Antibody (9E1) [Fluorescein] Summary

• Immunogen: Recombinant human P-Selectin
  Extracellular domain
• Specificity: This antibody binds to CHO cells transfected with human P-Selectin but not to CHO cells transfected with either human E-Selectin or human L-Selectin.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Selectin/CD62P Antibody (9E1) [Fluorescein]

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

Human P-Selectin, also known as GMP-140, LECAM-3, PADGEM, and CD62P, is a member of the Selectin family, and is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin Glycoprotein Ligand-1).

P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling' of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble P-Selectin are present in the biological fluids of apparently normal individuals.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Selectin/CD62P Antibody (BBA34)(5)

Publications using BBA34

• O Borst, P Münzer, N Alnaggar, S Geue, R Tegtmeyer, D Rath, M Droppa, P Seizer, S Heitmeier, JWM Heemskerk, LK Jennings, RF Storey, DJ Angiolillo, B Rocca, H Spronk, H Ten Cate, M Gawaz, T Geisler Inhibitory mechanisms of very low-dose rivaroxaban in non-ST-elevation myocardial infarction Blood Adv, 2018-03-27;2(6):715-730. 2018-03-27 [PMID: 29588304] (Flow Cytometry, Human)
• A Ojha, D Nandi, H Batra, R Singhal, GK Annarapu, S Bhattachar, T Seth, L Dar, GR Medigeshi, S Vrati, NK Vikram, P Guchhait Platelet activation determines the severity of thrombocytopenia in dengue infection Sci Rep, 2017-01-31;7(0):41697. 2017-01-31 [PMID: 28139770] (Flow Cytometry, Human)
• HbS Binding to GP1b? Activates Platelets in Sickle Cell Disease PLoS ONE, 2016-12-09;11(12):e0167899. 2016-12-09 [PMID: 27936141] (Flow Cytometry, Human)
• Gutierrez G, Mendoza C, Zapata E, Montiel A, Reyes E, Montano LF, Lopez-Marure R Dehydroepiandrosterone inhibits the TNF-alpha-induced inflammatory response in human umbilical vein endothelial cells. Atherosclerosis, 2006-03-30;190(1):90-9. 2006-03-30 [PMID: 16574124] (Flow Cytometry, Human)
• Ramachandran R, Morice AH, Compton SJ Proteinase-activated receptor2 agonists upregulate granulocyte colony-stimulating factor, IL-8, and VCAM-1 expression in human bronchial fibroblasts. Am. J. Respir. Cell Mol. Biol., 2006-02-23;35(1):133-41. 2006-02-23 [PMID: 16498082] (Flow Cytometry, Human)

Review for P-Selectin/CD62P Antibody (BBA34) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/20/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 21817096
• Species: Human

Bioinformatics

• Gene Symbol: SELP