P-Selectin/CD62P Antibody (127933) [Unconjugated]

• Reactivity: Mu
• Applications: WB, ELISA(Cap)
• Clone: 127933
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated

P-Selectin/CD62P Antibody (127933) [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse P-Selectin/CD62P
  Trp42-Ala709
  Accession # Q01102
• Specificity: Detects mouse P-Selectin/CD62P in ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) L-Selectin, rmE-Selectin, or recombinant human P-Selectin is observed.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Rat
• Gene: SELP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 1 ug/mL
• Application Notes: ELISA Detection: Mouse P-Selectin/CD62P Biotinylated Affinity Purified PAb (Catalog number BAF737) Standard: Recombinant Mouse P-Selectin/CD62P Fc Chimera (Catalog number 737-PS)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for P-Selectin/CD62P Antibody (127933) [Unconjugated]

• CD62P antigen
• CD62P
• FLJ45155
• GMP140
• GRMP
• PADGEM
• PADGEMantigen CD62)
• PSEL
• P-Selectin
• selectin P (granule membrane protein 140kDa, antigen CD62)
• SELP

Background

Mouse P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. P-Selectin is translocated to the cell surface within minutes, from alpha granules of platelets or Weibel-Palade bodies of endothelial cells, following stimulation with thrombin, histamine, PMA or peroxides. P-Selectin binds to a 106 kDa protein present on myeloid cells, neutrophils, monocytes and lymphocytes, termed PSGL-1 (P-Selectin glycoprotein ligand-1). P-Selectin plays a role in the adhesion of leukocytes and neutrophils to the endothelium. Acting in cooperation with L-Selectin, P-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic ‘rolling’ of the leukocytes on the endothelium. This initial interaction is followed by a stronger interaction involving E-Selectin, and later ICAM-1 and VCAM-1, that leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. Mouse P-Selectin cDNA encodes a 768 amino acid (aa) residue type I transmembrane protein with a 41 aa signal peptide, a 668 aa extracellular domain, a transmembrane domain and a short (35 aa) cytoplasmic domain. The extracellular domain has an NH 2 -terminal C-type lectin domain and an EGF-like domain followed by a series of complement factor A repeat homology domains. The extracellular domains of human and mouse P-Selectin share approximately 73% sequence homology.

1. Kansas, G.S. (1996) Blood 88:3259.
2. McEver, R.P. and R.D. Cummings (1997) J. Clin. Invest. 100:485.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for P-Selectin/CD62P Antibody (MAB737)(2)

Publications using MAB737

• A Chauhan, L Sheriff, MT Hussain, GJ Webb, DA Patten, EL Shepherd, R Shaw, CJ Weston, D Haldar, S Bourke, R Bhandari, S Watson, DH Adams, SP Watson, PF Lalor The platelet receptor CLEC-2 blocks neutrophil mediated hepatic recovery in acetaminophen induced acute liver failure Nat Commun, 2020-04-22;11(1):1939. 2020-04-22 [PMID: 32321925] (IHC, Mouse)
• Santen S, Schramm R, Menger MD, Wang Y, Jeppsson B, Thorlacius H P-selectin glycoprotein ligand-1 regulates chemokine-dependent leukocyte recruitment in colonic ischemia-reperfusion. Inflamm. Res., 2007-11-01;56(11):452-8. 2007-11-01 [PMID: 17891336] (Neutralization, Mouse)

Bioinformatics

• Gene Symbol: SELP
• Uniprot:
  • Q01102()