Osterix/Sp7 Antibody (764704) [Alexa Fluor® 405]

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: 764704
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 405

Osterix/Sp7 Antibody (764704) [Alexa Fluor® 405] Summary

• Immunogen: E. coli-derived recombinant human Osterix/Sp7
  Met19-Leu288
  Accession # Q8TDD2
• Specificity: Detects human Osterix/Sp7 in direct ELISAs.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osterix/Sp7 Antibody (764704) [Alexa Fluor® 405]

• MGC126598
• Osterix
• OSX
• OSXosterix
• Sp7 transcription factor
• Sp7
• transcription factor Sp7
• Zinc finger protein osterix

Background

Osterix, also known as Sp7, is an approximately 45 kDa transcription factor that is required for osteogenesis and bone homeostasis. Osterix cooperates with BMP-6 in the differentiation of osteoblasts from mesenchymal stem cells by regulating the transcription of several genes involved in osteoblast differentiation and function (i.e. SATB2, Collagen V, and SOST). The transcription of Osterix is induced by BMP-2, IGF-I, and parathyroid hormone, while its activity is regulated by Akt and p38 MAPK-mediated phoshorylation. Osterix contains a transactivation domain (aa 141-210) and three zinc finger domains (aa 294-318, aa 324-348, and aa 354-376). Alternative splicing generates a short isoform that lacks the N-terminal 18 amino acids. Within aa 19-288, human Osterix shares 94% aa sequence identity with mouse and rat Osterix.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.