Human Osteopontin/OPN Recombinant Protein

Product Discontinued

Human Osteopontin/OPN Recombinant Protein Summary

• Description: A biologically active protein corresponding to SPP1.
• Preparation Method: Lyophilized from 0.2 mm filtered concentrated solution in PBS.
• Details of Functionality: When immobilized, rhOsteopontin supports more than 30% human HEK-293 cell adhesion.
• Source: Mouse myeloma cell line
• Protein/Peptide Type: Recombinant Protein
• Gene: SPP1
• Endotoxin Note: Less than 1 EU/mg of the rHuOsteopontin as determined by LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Centrifuge prior to opening. Reconstitute with PBS to a final concentration of 0.1 mg/ml. Further dilutions should be made in appropriately buffered solutions.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human Osteopontin/OPN Recombinant Protein

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN), also known as SPP1, Eta-1, or BSP, is a highly acidic, 45-75 kDa secreted molecule in the SIBLING family of matricellular proteins. It can be variably modified with O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN binds several integrins and can bind additional integrins after it is proteolytically cleaved. OPN is prominently expressed in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell adhesion, and cell migration. OPN expression is upregulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and it contributes to the pathophysiology of these conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SPP1