Porcine Osteopontin/OPN ELISA Kit (Colorimetric)

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Porcine Osteopontin/OPN ELISA Kit (Colorimetric) - Standard Curve Reference

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP2-68078
    • Availability
      Product Discontinued

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Porcine Osteopontin/OPN ELISA Kit (Colorimetric) Summary

Specificity
This kit recognizes Porcine OPN in samples. No significant cross-reactivity or interference between Porcine OPN and analogues was observed.
Standard Curve Range
62.50 - 4000 pg/mL
Sensitivity
37.50 pg/mL
Assay Type
Sandwich-ELISA
Inter-Assay
CV% < 5.3%
Intra-Assay
CV% < 5.15%
Spike Recovery
87-105%
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
SPP1

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Biotinylated Detection Ab Diluent
  2. Concentrated Biotinylated Detection Ab (100x)
  3. Concentrated HRP Conjugate (100x)
  4. Concentrated Wash Buffer (25x)
  5. HRP Conjugate Diluent
  6. Micro ELISA Plate (Dismountable)
  7. Plate Sealer
  8. Product Manual
  9. Reference Standard
  10. Sample Diluent
  11. Stop Solution
  12. Substrate Reagent

Alternate Names for Porcine Osteopontin/OPN ELISA Kit (Colorimetric)

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

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Product General Protocols

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FAQs for Osteopontin/OPN ELISA Kit (NBP2-68078). (Showing 1 - 1 of 1 FAQ).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Bioinformatics

Gene Symbol SPP1