Human Osteopontin/OPN Recombinant Protein

Product Discontinued

Human Osteopontin/OPN Recombinant Protein Summary

• Description: A biologically active protein with a C-terminal 6-His tag corresponding to amino acids Ile17 - Asn300 to SPP1.
  
  

  Source: Mouse myeloma cell line, NS0

• Preparation Method: Lyophilized from a 0.2 um filtered solution in PBS.
• Details of Functionality: Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. Recombinant Human (rh) Osteopontin/OPN immobilized at 1 ug/mL, 100 uL/well, will mediate the adhesion of added 293 cells >30% (1 x 105 cells/well). Recombinant Human Coagulation Factor II/Thrombin (Catalog # 1473-SE) proteolytic treatment of this rhOsteopontin/his can increase HEK293 cell adhesion by about 5-fold.
• Source: Nso cells
• Protein/Peptide Type: Recombinant Protein
• Gene: SPP1
• Endotoxin Note: <0.10 EU per 1 ug of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 32.9 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute with 0.1 mg/ml in sterilized PBS containing at least 0.1% human or BSA to desired concentration.

Notes

This lyophilized preparation is stable at 2-8 degrees C, but should be kept at -20 degrees C for long term storage, preferably desiccated. Upon reconstitution, the preparation is most stable at -20 to -80 degrees C, and can be stored for one week at 2-8 degrees C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 degrees C to -80 degrees C. Avoid repeated freeze/thaw cycles.

Alternate Names for Human Osteopontin/OPN Recombinant Protein

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: SPP1