Osteopontin/OPN Antibody (53) - BSA Free

Western Blot: Osteopontin/OPN Antibody (53) [NBP1-19184] - MDA-MB-435 cell lysates, conditioned medium from MDA-MB-435 cells containing secreted OPN or recombinant protein.

Immunohistochemistry-Paraffin: Osteopontin/OPN Antibody (53) [NBP1-19184] - Analysis of formalin-fixed, paraffin-embedded breast cancer tissue stained with 2 ug/ml anti-Osteopontin (53).

Product Discontinued

Osteopontin/OPN Antibody (53) - BSA Free Summary

• Immunogen: Recombinant human Osteopontin
• Specificity: Osteopontin (53)
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPP1
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Osteopontin/OPN Antibody (53) - BSA Free

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN) is an acidic extracellular matrix cell adhesion protein that is relatively abundant not only in bone matrix, plasma, urine, and milk, but is also found in malignant and atherogenic tissues. Phosphorylation, glycosylation and calcium modifications allow intact and fragmented Osteopontin to direct a variety of diverse responses including tissue remodeling, inflammation and cell survival. Plasma Osteopontin has been shown to provide prognostic information in breast, prostate, colon, and lung cancers as well as metastatic carcinomas. The notable presence of Osteopontin in a variety of tumors is strongly correlated to pathological stage, suggesting its critical role in tumor invasiveness, progression and metastasis. In addition, Osteopontin inhibits inducible nitric oxide synthase activity, thereby protecting tumor cells from NO-mediated macrophage cytotoxic attack. Osteopontin is found in atherosclerotic plaques and may drive a number of diabetic vascular pathologies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SPP1
• Entrez:
  • 6696(Human)
• Uniprot:
  • p10451(Human)