Osteopontin/OPN Antibody (223112)

• Reactivity: Hu
• Applications: ELISA
• Clone: 223112
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Osteopontin/OPN Antibody (223112) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Osteopontin
  Ile17-Asn300
  Accession # NP_000573.1
• Specificity: Detects human Osteopontin in ELISAs. In ELISAs, this antibody does not cross-react with recombinant mouse Osteopontin.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: SPP1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Standard (Matched Pair)
  • ELISA 2-8 ug/mL
• Application Notes: ELISA Capture: Human Osteopontin/OPN Antibody (Catalog # MAB14332)
  ELISA Detection: Human Osteopontin/OPN Biotinylated Antibody (Catalog # BAF1433)
  Standard: Recombinant Human Osteopontin/OPN (Catalog # 1433-OP)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteopontin/OPN Antibody (223112)

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN, previously also referred to as transformation-associated secreted phosphoprotein, bone sialoprotein I, 2ar, 2B7, early T lymphocyte activation 1 protein, minopotin, calcium oxalate crystal growth inhibitor protein), is a secreted, highly acidic, calcium-binding, RGD-containing, phosphorylated glycoprotein originally isolated from bone matrix (1). Subsequently, OPN has been found in kidney, placenta, blood vessels and various tumor tissues. Many cell types (including macrophages, osteoclasts, activated T-cells, fibroblasts, epithelial cells, vascular smooth muscle cells, and natural killer cells) can express OPN in response to activation by cytokines, growth factors or inflammatory mediators. Elevated expression of OPN has also been associated with numerous pathobiological conditions such as atherosclerotic plaques, renal tubulointerstitial fibrosis, granuloma formations in tuberculosis and silicosis, neointimal formation associated with balloon catheterization, metastasizing tumors, and cerebral ischemia. Human OPN cDNA encodes a 314 amino acid (aa) residue precursor protein with a 16 aa residue predicted signal peptide that is cleaved to yield a 298 aa residue mature protein with an integrin binding sequence (RGD), and N- and O-glycosylation sites. By alternative splicing, at least three human OPN isoforms exist. OPN has been shown to bind to different cell types through RGD-mediated interaction with the integrins alpha _v beta ₁, alpha _v beta ₃, alpha _v beta ₅, and non-RGD-mediated interaction with CD44 and the integrins alpha ₈ beta ₁ or alpha ₉ beta ₁. OPN exists both as a component of extracellular matrix and as a soluble molecule. Functionally, OPN is chemotactic for macrophages, smooth muscle cells, endothelial cells and glial cells. OPN has also been shown to inhibit nitric oxide production and cytotoxicity by activated macrophages. Human, mouse, rat, pig and bovine OPN share from approximately 40% - 80% amino acid sequence identity. Osteopontin is a substrate for proteolytic cleavage by thrombin, enterokinase, MMP-3 and MMP-7. The functions of OPN in a variety of cell types were shown to be modified as a result of proteolytic cleavage (2, 3).

1. Ann. N.Y. Acad. Sci. (1995) 760, Apr. 21.
2. Senger, D.R. et al. (1996) Biochim. Biophys. Acta. 1314:13.
3. Agnihotri, R. et al. (2001) J. Biol. Chem. 276:28261.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Osteopontin/OPN Antibody (MAB14332)(1)

Publication using MAB14332

• J Song, SL Merbs, LJ Sokoll, DW Chan, Z Zhang A multiplex immunoassay of serum biomarkers for the detection of uveal melanoma Clin Proteomics, 2019-03-05;16(0):10. 2019-03-05 [PMID: 30867659] (Human)

Bioinformatics

• Gene Symbol: SPP1
• Uniprot:
  • NP_000573.1()