Osteopontin/OPN Antibody (190312) [Unconjugated]

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Osteopontin/OPN was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-Human Osteopontin/OPN Monoclonal Antibody (Catalog # MAB1433) at 25 µg/mL overnight at 4 °C. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
190312
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price

Osteopontin/OPN Antibody (190312) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Osteopontin
Ile17-Asn300
Accession # NP_000573.1
Specificity
Detects human Osteopontin in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SPP1
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL
Control
Osteopontin/OPN Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
MAB1433 in the following application:

Publications
Read Publications using
MAB1433 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Osteopontin/OPN Antibody (190312) [Unconjugated]

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin (OPN), previously called SPP1 (secreted phosphoprotein 1), Eta1(early T lymphocyte activation 1) or BSP (bone sialoprotein), is a secreted molecule in the SIBLING (small integrin binding ligand N-linked glycoprotein) family of noncollagenous matricellular proteins (1‑3). Human OPN is synthesized as a 317 aminoacid (aa) precursor protein with a 16 aa signal peptide and a 301 aa mature protein (3). Mature human OPN shares 64% and 62% aa sequence identity with mouse and rat OPN, respectively. OPN is highly acidic and has 26 potential Ser/Thr phosphorylation sites and a C‑terminal CD44 binding site (1‑4). Depending on tissue-specific modification by O- and N‑glycosylation, sulfation, phosphorylation and transglutamination, OPN can be detected at 45‑75 kDa (5, 6). The central region of OPN contains RGD and nonRGD binding sites for multiple integrins (3, 4). Adjacent to the RGD motif is the sequence SVVYGLR (SLAYGLR in mouse) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by thrombin in tumors and sites of tissue injury
(6‑8). OPN can also be cleaved by MMP3, 7, 9, and 12 within the SVVYGLR motif and at sites closer to the C‑terminus (8, 9). OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration (1, 2, 4, 6). Its expression is up‑regulated during inflammation, obesity, atherosclerosis, cancer, and tissue damage, and contributes to the pathophysiology of these conditions (1, 2, 6, 9, 10).
  1. Scatena, M. et al. (2007) Arterioscler. Thromb. Vasc. Biol. 27:2302.
  2. Rangaswami, H. et al. (2006) Trends Cell Biol. 16:79.
  3. Young, M.F. et al. (1990) Genomics 7:491.
  4. Weber, G.F. et al. (2002) J. Leukoc. Biol. 72:752.
  5. Keykhosravani, M. et al. (2005) Biochemistry 44:6990.
  6. Kazanecki, C.C. et al. (2007) J. Cell. Biochem. 102:912.
  7. Senger, D.R. et al. (1994) Mol. Biol. Cell 5:565.
  8. Yokosaki, Y. et al. (2005) Matrix Biol. 24:418.
  9. Takafuji, V. et al. (2007) Oncogene 26:6361.
  10. Kiefer, F.W. et al. (2010) Diabetes 59:935.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Osteopontin/OPN Antibody (MAB1433)(3)

Review for Osteopontin/OPN Antibody (MAB1433) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using MAB1433:
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IHC-P
(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 02/10/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 20562127
SpeciesHuman

FAQs for Osteopontin/OPN Antibody (MAB1433) (0)

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Control Lysate(s)

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Recent Reviews

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1
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Anonymous
02/10/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol SPP1
Uniprot