Osteocalcin Antibody - BSA Free

Western Blot: Osteocalcin Antibody [NBP3-13133] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 15% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Western Blot: Osteocalcin Antibody [NBP3-13133] - Recombinant Osteocalcin were separated by 10% SDS-PAGE, and the membrane was blotted with Osteocalcin antibody (NBP3-13133) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

ELISA: Osteocalcin Antibody [NBP3-13133] - Indirect ELISA analysis was performed by coating plate with 50 uL of recombinant full-length Osteocalcin protein at concentrations ranging from 0.938 ng/mL to 60 ng/mL. The coated protein is detected with Osteocalcin antibody (NBP3-13133) at 1 ug/mL.

• Reactivity: Hu, Po, Bv, Pm, RM, Sh
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Osteocalcin Antibody - BSA Free Summary

• Description: Centrifuge briefly prior to opening.
• Immunogen: Recombinant protein encompassing a sequence within the Center region of human Osteocalcin. The exact sequence is proprietary.
• Predicted Species: Porcine (91%), Chimpanzee (97%), Bovine (91%), Rhesus Macaque (95%), Sheep (91%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: BGLAP
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:1000-1:10000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 11 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Chicken (81%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Osteocalcin Antibody - BSA Free

• BGLAP
• BGP
• bone gamma-carboxyglutamate (gla) protein (osteocalcin)
• bone gamma-carboxyglutamate (gla) protein
• Bone Gla protein
• Gamma-carboxyglutamic acid-containing protein
• OC
• OCN
• Osteocalcin

Background

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BGLAP