OS9 Antibody

Images

 
Genetic Strategies: Knockdown Validated: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by ...read more
Western Blot: OS9 Antibody [NB100-519] - Detection of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2 by western analysis.
Immunohistochemistry: OS9 Antibody [NB100-519] - Staining of OS9 in mouse smooth muscle.
Immunoprecipitation: OS9 Antibody [NB100-519] - Immunoprecipitation of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP, KD
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml
Validated by:
     

Genetic Strategies

   

OS9 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of isoform 1 of the human OS9 protein (within residues 300-400). [Swiss-Prot# Q13438]
Specificity
This is specific for isoforms 1 and 2 of human OS-9.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OS9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 0.2-1.0 ug/ml
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 0.2-1.0 ug/ml
Application Notes
This OS9 antibody is useful for Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections, Immunoprecipitation and Western blot. In WB, bands can be seen at ~75 kDa representing isoform 1 and at ~70 kDa representing isoform 2.
Control
OS9 Overexpression Lysate
Control Peptide
OS9 Peptide (NB100-519PEP)
Publications
Read Publications using
NB100-519 in the following applications:

  • 1 publication
  • 1 publication
  • IP
    1 publication
  • WB
    6 publications

Reactivity Notes

Human and mouse.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.01% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OS9 Antibody

  • Amplified in osteosarcoma 9
  • endoplasmic reticulum lectin 2
  • ERLEC2
  • erlectin 2
  • OS-9
  • osteosarcoma amplified 9, endoplasmic reticulum associated protein
  • osteosarcoma amplified 9, endoplasmic reticulum lectin
  • protein OS-9

Background

OS9 (protein OS-9 or amplified in osteosarcoma 9) is a lectin/glycoprotein that functions in ER quality control and ER-associated degradation (ERAD pathway) of newly synthesized proteins. TRPV4 is its major target and OS9 also interact with GRP94, DERL2, HSP90B1, CREB3, ERLEC1, HSPA5 etc., forms a part of HRD1 ubiquitin ligase complex (SYVN1/HRD1 and SEL1L) which is involved in retrotranslocation of misfolded proteins from ER to cytosol for ubiquitin dependent degradation. OS9 is essential for recognizition of terminally misfolded non-glycosylated proteins or improperly folded glycoproteins, binding with them for their retention in ER, and transferring them to ubiquitination machinery for degradation. Moreover, it has also been shown to specifically protect some proteins from degradation process before maturation, ensuring their biological functionality. Because OS9 is implicated in protein quality control, it is closely related to ER stress, and gets up-regulated in response to ER stress. Recently, it has been shown to highly express in adipocytes wherein it regulated lipid storage, and its overexpression is also observed in sarcomas especially in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OS9 Antibody (NB100-519)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ICC/IF, IHC-P, IP, WB.


Filter By Application
ICC/IF
(1)
IHC-P
(1)
IP
(1)
WB
(6)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB100-519 Applications Species
Chen S, Yin C, Lao T et al. AMPK-HDAC5 pathway facilitates nuclear accumulation of HIF-1alpha and functional activation of HIF-1 by deacetylating Hsp70 in the cytosol. Cell Cycle 2015 Aug 3 [PMID: 26061431] (WB) WB
Koenig Pa, Nicholls Pk, Schmidt Fi et al. The E2 Ubiquitin Conjugating Enzyme UBE2J1 is Required for Spermiogenesis in Mice. J. Biol. Chem. 2014 Oct 15 [PMID: 25320092] (WB, Mouse) WB Mouse
Sun Yw, Chen Yf, Li J et al. A novel long non-coding RnA EnST00000480739 suppresses tumour cell invasion by regulating OS-9 and HIF-1Alpha in pancreatic ductal adenocarcinoma. Br. J. Cancer. 2014 Oct 14 [PMID: 25314054] (WB, IHC-P, Human) WB, IHC-P Human
Tang HY, Huang CH, Zhuang YH et al. EDEM2 and OS-9 Are Required for ER-Associated Degradation of Non-Glycosylated Sonic Hedgehog PLoS ONE. 2014 Jun 10 [PMID: 24910992] (WB, Human)

Details:
OS9 antibody used for WB on Human 293T cells stabely expressing N278A-HA precursor that were depleted of OS-9 by siRNA (Figure 6A). Also, SHH-HA or N278A-HA plasmics were co-transfected with either vector, wild type OS-9.2 (WT-OS-9.2), or R188A-OS-9 mutants and HA immunoprecipitants from lysed cells were processed for WB with OS-9 antibody (Figure 6C).
WB Human
Huang CH, Hsiao HT, Chu YR et al. Derlin2 facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum. J Biol Chem 2013 Jul 18 [PMID: 23867461] (WB, Human) WB Human
Hofmann S, Fischer A, Nothnagel M et al. Genome-wide association analysis reveals 12q13 3-q14 1 as new risk locus for sarcoidosis. Eur Respir J 2013 Apr [PMID: 22936702]

Details:
Using the Biotin conjugated version of NB100-519, catalog number NB100-519B.
Bernasconi R, Pertel T, Luban J et al. A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal. J Biol Chem;283(24):16446-54. 2008 Jun 13. [PMID: 18417469] (IP, WB, ICC/IF, Human) IP, WB, ICC/IF Human

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for OS9 Antibody (NB100-519)

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Blogs on OS9.

OS9: Taking proteins to the ER finish line
The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no...  Read full blog post.

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Bioinformatics

Gene Symbol OS9
Entrez
Uniprot