OS9 Antibody - BSA Free

Western Blot: OS9 Antibody [NB100-519] - Contribution and interaction of OS-9 for degradation of SHH proteins. Cells stably expressing the N278A-HA precursor were depleted of OS-9 by siRNA, and the fate of N278A was followed after cycloheximide (CHX) addition. All samples were analyzed by SDS-PAGE and immunoblotting with HA antibodies. Controls were treated with a commercial negative control siRNA. The samples were also immunoblotted for OS-9 to show the extent of depletion. Immunoblotting with GAPDH antibodies served as loading control. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0092164) licensed under a CC-BY license.

Western Blot: OS9 Antibody [NB100-519] - Detection of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2 by western analysis.

Immunohistochemistry: OS9 Antibody [NB100-519] - Staining of OS9 in mouse smooth muscle.

Immunoprecipitation: OS9 Antibody [NB100-519] - Immunoprecipitation of OS-9.1 and OS-9.2 in total HEK293 cells, HEK293 cells expressing OS-9.1 or OS-9.2.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

OS9 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit OS9 Antibody - BSA Free (NB100-519) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-OS9 Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of isoform 1 of the human OS9 protein (within residues 300-400). [Swiss-Prot# Q13438]
• Specificity: This is specific for isoforms 1 and 2 of human OS-9.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OS9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.2-1.0 ug/ml
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Paraffin 1:100-1:250
  • Immunoprecipitation 1:10-1:500
  • Knockdown Validated
  • Western Blot 0.2-1.0 ug/ml
• Application Notes: In WB, bands can be seen at ~75 kDa representing isoform 1 and at ~70 kDa representing isoform 2.
• Control:

  OS9 Overexpression Lysate

• Control Peptide:

  OS9 Peptide (NB100-519PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.01% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for OS9 Antibody - BSA Free

• Amplified in osteosarcoma 9
• endoplasmic reticulum lectin 2
• ERLEC2
• erlectin 2
• OS-9
• osteosarcoma amplified 9, endoplasmic reticulum associated protein
• osteosarcoma amplified 9, endoplasmic reticulum lectin
• protein OS-9

Background

OS9 (protein OS-9 or amplified in osteosarcoma 9) is a lectin/glycoprotein that functions in ER quality control and ER-associated degradation (ERAD pathway) of newly synthesized proteins. TRPV4 is its major target and OS9 also interact with GRP94, DERL2, HSP90B1, CREB3, ERLEC1, HSPA5 etc., forms a part of HRD1 ubiquitin ligase complex (SYVN1/HRD1 and SEL1L) which is involved in retrotranslocation of misfolded proteins from ER to cytosol for ubiquitin dependent degradation. OS9 is essential for recognizition of terminally misfolded non-glycosylated proteins or improperly folded glycoproteins, binding with them for their retention in ER, and transferring them to ubiquitination machinery for degradation. Moreover, it has also been shown to specifically protect some proteins from degradation process before maturation, ensuring their biological functionality. Because OS9 is implicated in protein quality control, it is closely related to ER stress, and gets up-regulated in response to ER stress. Recently, it has been shown to highly express in adipocytes wherein it regulated lipid storage, and its overexpression is also observed in sarcomas especially in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OS9 Antibody (NB100-519)(7)

Publications using NB100-519

• Chen S, Yin C, Lao T et al. AMPK-HDAC5 pathway facilitates nuclear accumulation of HIF-1alpha and functional activation of HIF-1 by deacetylating Hsp70 in the cytosol. Cell Cycle 2015-08-03 [PMID: 26061431] (WB)
• Koenig Pa, Nicholls Pk, Schmidt Fi et al. The E2 Ubiquitin Conjugating Enzyme UBE2J1 is Required for Spermiogenesis in Mice. J. Biol. Chem. 2014-10-15 [PMID: 25320092] (WB, Mouse)
• Sun Yw, Chen Yf, Li J et al. A novel long non-coding RnA EnST00000480739 suppresses tumour cell invasion by regulating OS-9 and HIF-1Alpha in pancreatic ductal adenocarcinoma. Br. J. Cancer. 2014-10-14 [PMID: 25314054] (WB, IHC-P, Human)
• Tang HY, Huang CH, Zhuang YH et al. EDEM2 and OS-9 Are Required for ER-Associated Degradation of Non-Glycosylated Sonic Hedgehog PLoS ONE. 2014-06-10 [PMID: 24910992] (WB, Human)
  
  Details:
  OS9 antibody used for WB on Human 293T cells stabely expressing N278A-HA precursor that were depleted of OS-9 by siRNA (Figure 6A). Also, SHH-HA or N278A-HA plasmics were co-transfected with either vector, wild type OS-9.2 (WT-OS-9.2), or R188A-OS-9 mutants and HA immunoprecipitants from lysed cells were processed for WB with OS-9 antibody (Figure 6C).
• Huang CH, Hsiao HT, Chu YR et al. Derlin2 facilitates HRD1-mediated retro-translocation of sonic hedgehog at the endoplasmic reticulum. J Biol Chem 2013-07-18 [PMID: 23867461] (WB, Human)
• Hofmann S, Fischer A, Nothnagel M et al. Genome-wide association analysis reveals 12q13 3-q14 1 as new risk locus for sarcoidosis. Eur Respir J 2013-04-01 [PMID: 22936702]
  
  Details:
  Using the Biotin conjugated version of NB100-519, catalog number NB100-519B.
• Bernasconi R, Pertel T, Luban J et al. A dual task for the Xbp1-responsive OS-9 variants in the mammalian endoplasmic reticulum: inhibiting secretion of misfolded protein conformers and enhancing their disposal. J Biol Chem;283(24):16446-54. 2008-06-13 [PMID: 18417469] (WB, ICC/IF, IP, Human)

Bioinformatics

• Gene Symbol: OS9
• Entrez:
  • 10956(Human)
  • 216440(Mouse)
• Uniprot:
  • Q13438(Human)