OS9 Antibody [Alexa Fluor™ Plus 488]

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC, IP, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor Plus 488

OS9 Antibody [Alexa Fluor™ Plus 488] Summary

• Immunogen: Synthetic peptide made to an internal portion of isoform 1 of the human OS9 protein (within residues 300-400). [Swiss-Prot# Q13438]
• Specificity: This is specific for isoforms 1 and 2 of human OS-9.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OS9
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Knockdown Validated
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for OS9 Antibody [Alexa Fluor™ Plus 488]

• Amplified in osteosarcoma 9
• endoplasmic reticulum lectin 2
• ERLEC2
• erlectin 2
• OS-9
• osteosarcoma amplified 9, endoplasmic reticulum associated protein
• osteosarcoma amplified 9, endoplasmic reticulum lectin
• protein OS-9

Background

OS9 is a lectin which functions in endoplasmic reticulum quality control and ER-associated degradation. This ER glycoprotein is found in the intralumenal level and highly expressed in tumor tissue. The OS9 gene is transcriptionally induced upon activation of the Ire1/Xbp1 ER-stress pathway.

Functionally, OS9 binds to HIF-1, a key regulator of the hypoxic response and angiogenesis, and promotes the degradation of one of its subunits. It may also bind terminally misfolded non-glycosylated proteins as well as improperly folded glycoproteins, retain them in the ER, and possibly transfer them to the ubiquitination machinery and promote their degradation. One such possible target includes TRPV4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OS9