OS9 Antibody

Images

 
Western Blot: OS9 Antibody [BC100-520] - WB analysis of OS9 in 1. A431 cell lysate, 2. HepG2 cell lysate, 3. MCF7 cell lysate and 4. 3T3 cell lysate.
Immunocytochemistry/ Immunofluorescence: OS9 Antibody [BC100-520] - OS9 antibody was tested in HeLa cells with FITC (green). Nuclei and alpha-tubulin were counterstained with Dapi (blue) and Dylight 550 (red).
Simple Western: OS9 Antibody [BC100-520] - Simple Western lane view shows a specific band for OS9 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

OS9 Antibody Summary

Immunogen
A C-terminal synthetic peptide made to isoform 1 of the human OS9 protein sequence (between residues 600-667). There are 3 isoforms of this protein. The immunogen used for this antibody is conserved in isoforms 1, 2, and 3 of the human OS9 protein.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
OS9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:100
  • Immunocytochemistry/Immunofluorescence 1:500
Application Notes
This OS9 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot, where a band can be seen at approx. 75 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
OS9 Overexpression Lysate
OS9 Knockout 293T Cell Lysate
Control Peptide
Reviewed Applications
Read 1 Review rated 4
using
BC100-520 in the following applications:

Publications
Read Publications using
BC100-520 in the following applications:

  • WB
    4 publications

Reactivity Notes

Human, mouse and rat (see customer review).

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Glycine and 0.15M NaCl
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Immunogen affinity purified

Alternate Names for OS9 Antibody

  • Amplified in osteosarcoma 9
  • endoplasmic reticulum lectin 2
  • ERLEC2
  • erlectin 2
  • OS-9
  • osteosarcoma amplified 9, endoplasmic reticulum associated protein
  • osteosarcoma amplified 9, endoplasmic reticulum lectin
  • protein OS-9

Background

OS9 (protein OS-9 or amplified in osteosarcoma 9) is a lectin/glycoprotein that functions in ER quality control and ER-associated degradation (ERAD pathway) of newly synthesized proteins. TRPV4 is its major target and OS9 also interact with GRP94, DERL2, HSP90B1, CREB3, ERLEC1, HSPA5 etc., forms a part of HRD1 ubiquitin ligase complex (SYVN1/HRD1 and SEL1L) which is involved in retrotranslocation of misfolded proteins from ER to cytosol for ubiquitin dependent degradation. OS9 is essential for recognizition of terminally misfolded non-glycosylated proteins or improperly folded glycoproteins, binding with them for their retention in ER, and transferring them to ubiquitination machinery for degradation. Moreover, it has also been shown to specifically protect some proteins from degradation process before maturation, ensuring their biological functionality. Because OS9 is implicated in protein quality control, it is closely related to ER stress, and gets up-regulated in response to ER stress. Recently, it has been shown to highly express in adipocytes wherein it regulated lipid storage, and its overexpression is also observed in sarcomas especially in osteosarcoma OSA-CL and rhabdomyosarcoma RH30 cell lines.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Simple Western, ICC/IF

Publications for OS9 Antibody (BC100-520)(4)

Review for OS9 Antibody (BC100-520) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using BC100-520:
Filter by Applications
WB
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Riccardo Bernasconi
WB 07/04/2012
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Summary

ApplicationWestern Blot
FileView PDF

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OS9 Antibody (BC100-520) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Blogs on OS9.

OS9: Taking proteins to the ER finish line
The OS9 protein is a lectin/glycoprotein that maintains endoplasmic reticulum (ER) quality control and ER-associated degradation (the so-called ERAD pathway) of newly synthesized proteins. It is essential for the recognition of terminally misfolded no...  Read full blog post.

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Recent Reviews

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Riccardo Bernasconi
07/04/2012
Application: WB
Species:

Bioinformatics

Gene Symbol OS9
Entrez
Uniprot