ORP8 Antibody - BSA Free

Western Blot: ORP8 Antibody [NBP2-19634] - Sample (30 ug of whole cell lysate) A: U87-MG 7. 5% SDS PAGE gel, diluted at 1:10000.

Immunocytochemistry/Immunofluorescence: ORP8 Antibody [NBP2-19634] - Immunofluorescence analysis of methanol-fixed HeLa, using antibody at 1:500 dilution.

Western Blot: ORP8 Antibody [NBP2-19634] -Boiled and unboiled HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ORP8 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

ORP8 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit ORP8 Antibody - BSA Free (NBP2-19634) is a polyclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human ORP8. The exact sequence is proprietary.
• Predicted Species: Mouse (96%), Rat (97%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OSBPL8
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Western Blot 1:5000-1:20000
• Theoretical MW: 101 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for ORP8 Antibody - BSA Free

• MGC126578
• MGC133203
• MST120
• MSTP120
• ORP-8
• ORP8KIAA1451
• OSBP10DKFZp686A11164
• OSBP-related protein 8
• oxysterol binding protein-like 8
• oxysterol-binding protein-related protein 8

Background

This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Like most members, the encoded protein contains an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: OSBPL8
• Uniprot:
  • Q9BZF1(Human)