ORP1 Antibody

Images

 
Western Blot: ORP1 Antibody [NB100-1331] - (0.5ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Western Blot: ORP1 Antibody [NB100-1331] - Analysis of ORP1 in Human Muscle lysate using NB100-1331 at 0.5 ug/ ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

ORP1 Antibody Summary

Immunogen
Peptide with sequence C-YWDRNYFNLPDIY corresponding to C-Terminus according to NP_542164.2.
Specificity
This antibody is expected to recognise both reported human isoforms of this protein.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
OSBPL1A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
Application Notes
Western blot: Approx 48kDa band observed in Human Muscle lysate (predicted MW of 50kDa according to NP_060500).
IHC: Paraffin embedded Human Brain (Cerebellum) and Human Skeletal Muscle.
Positive Control
Skeletal Muscle Lysate (NB820-59253)
Publications
Read Publications using
NB100-1331 in the following applications:

  • WB
    2 publications

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ORP1 Antibody

  • FLJ10217
  • ORP-1oxysterol-binding protein-related protein 1 variant 1
  • ORP1oxysterol-binding protein-related protein 1
  • OSBP8
  • OSBPL1
  • OSBPL1B
  • OSBP-related protein 1
  • oxysterol binding protein-like 1A
  • oxysterol binding protein-like 1B
  • oxysterol-binding protein-related protein 1 variant 2

Background

Oxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ORP1 Antibody (NB100-1331)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for ORP1 Antibody (NB100-1331)

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Bioinformatics

Gene Symbol OSBPL1A
Entrez
Uniprot