ORP1 Antibody

Western Blot: ORP1 Antibody [NB100-1331] - (0.5ug/ml) of Human Muscle lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Skeletal Muscle. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Western Blot: ORP1 Antibody [NB100-1331] - Analysis of ORP1 in Human Muscle lysate using NB100-1331 at 0.5 ug/ ml. Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Immunohistochemistry-Paraffin: ORP1 Antibody [NB100-1331] - (5ug/ml) staining of paraffin embedded Human Cerebellum. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt, Bv, Ca
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

ORP1 Antibody Summary

• Description: Novus Biologicals Goat ORP1 Antibody (NB100-1331) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-ORP1 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-YWDRNYFNLPDIY corresponding to C-Terminus according to NP_542164.2.
• Specificity: This antibody is expected to recognise both reported human isoforms of this protein.
• Predicted Species: Rat (100%), Canine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: OSBPL1A
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.5-2 ug/ml
• Application Notes: Western blot: Approx 48kDa band observed in Human Muscle lysate (predicted MW of 50kDa according to NP_060500).
  IHC: Paraffin embedded Human Brain (Cerebellum) and Human Skeletal Muscle.
• Control:

  Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 22241858).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for ORP1 Antibody

• FLJ10217
• ORP-1oxysterol-binding protein-related protein 1 variant 1
• ORP1oxysterol-binding protein-related protein 1
• OSBP8
• OSBPL1
• OSBPL1B
• OSBP-related protein 1
• oxysterol binding protein-like 1A
• oxysterol binding protein-like 1B
• oxysterol-binding protein-related protein 1 variant 2

Background

Oxysterols are oxygenated derivatives of cholesterol that have a number of biological effects and play a key role in the maintenance of the body cholesterol balance. ORP1 is a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors that are believed to transport sterols from lysosomes to the nucleus where the sterol down-regulates the genes for the LDL receptor, HMG-CoA reductase, and HMG synthetase.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ORP1 Antibody (NB100-1331)(3)

Publications using NB100-1331

• Storey SM, McIntosh AL, Huang H et al. Intracellular cholesterol-binding proteins enhance HDL-mediated cholesterol uptake in cultured primary mouse hepatocytes Am J Physiol Gastrointest Liver Physiol 2012-04-15 [PMID: 22241858] (WB, Mouse)
• Shah, AH et al. Adenovirus RID{alpha} regulates endosome maturation by mimicking GTP-Rab7 J Cell Biol 2007-01-01 [PMID: 18039930] (WB)
  
  Details:
  This citation used the FITC version of this antibody.
• Lehto M, Laitinen S, Chinetti G et al. The OSBP-related protein family in humans. J Lipid Res 2001-08-01 [PMID: 11483621]

Bioinformatics

• Gene Symbol: OSBPL1A
• Entrez:
  • 114876(Human)
  • 64291(Mouse)
  • 259221(Rat)
• Uniprot:
  • Q9BXW6(Human)