Glutathione Peroxidase 3/GPX3 Antibody

Images

 
Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - GPX3 expressions in adenocarcinoma (AC). EnVision immunohistochemistry, original magnification x200. GPX3 positive reaction was mainly ...read more
Immunohistochemistry: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - GPX3 expressions in squamous carcinomas/adenosquamous carcinomas (SC/ASC). EnVision immunohistochemistry, original magnification x200. GPX3 ...read more
Western Blot: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - Analysis of GPX3 in human kidney lysate.
Immunohistochemistry-Paraffin: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human kidney using 1:200 conc. of Glutathione Peroxidase 3 antibody on a Bond ...read more
Immunocytochemistry/ Immunofluorescence: Glutathione Peroxidase 3/GPX3 Antibody [NBP1-06398] - The GPX3 antibody was tested in HeLa cells at a 1:250 dilution against Dylight 488 (Green). Alpha-tubulin and nuclei were ...read more

Product Details

Summary
Reactivity Hu, Rt, PmSpecies Glossary
Applications WB, IB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

Glutathione Peroxidase 3/GPX3 Antibody Summary

Immunogen
Full length human Glutathione Peroxidase 3 expressed in E. coli. [Swiss-Prot# P22352]
Localization
Secreted.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GPX3
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:200
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
Application Notes
This Glutathione Peroxidase 3 antibody is for Immunocytochemistry/Immunofluorescence and Western blot, where a band is seen ~25 kDa. Use in Immunohistochemistry-Paraffin reported in scientific literature (PMID 24167362). Use in immunoblotting reported in scientific literature (PMID: 29571702). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
25 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
HeLa Whole Cell Lysate
Publications
Read Publications using
NBP1-06398 in the following applications:

  • 1 publication
  • WB
    3 publications

Reactivity Notes

Human, monkey and rat. Immunogen sequence has 89% identity to mouse, 88% identity to bovine and 84% identity to porcine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Whole antisera
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for Glutathione Peroxidase 3/GPX3 Antibody

  • EC 1.11.1
  • EC 1.11.1.9
  • Extracellular glutathione peroxidase
  • glutathione peroxidase 3 (plasma)
  • Glutathione Peroxidase 3
  • GPX3
  • GPx-3
  • GPXP
  • GPX-P
  • GSHPx-3
  • GSHPx-P
  • Plasma glutathione peroxidase

Background

GPX3 (Glutathione Peroxidase 3) belongs to the glutathione peroxidase family. It protects cells and enzymes from oxidative damage by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide by glutathione. There is increasing evidence that GPX3 is a novel tumor suppressor and a therapeutic target for insulin resistance and diabetes mellitus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutathione Peroxidase 3/GPX3 Antibody (NBP1-06398)(5)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Glutathione Peroxidase 3/GPX3 Antibody (NBP1-06398) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GPX3
Entrez
Uniprot