Opsin 1 (Medium Wave) Antibody - BSA Free

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Chicken
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Opsin 1 (Medium Wave) Antibody - BSA Free Summary

• Immunogen: Synthetic peptides from mouse green opsin and zebra finch red opsin.
• Specificity: Opsin, red and green. The antibody reacts with a protein of ~40 kDa.
• Clonality: Polyclonal
• Host: Chicken
• Gene: OPN1MW
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Western Blot 1:500:1:2000
• Application Notes: ImmunoBlotting: on mouse brain extract. To minimize background staining it is suggested that you include a higher content of detergent (Tween-20) in your dilution and washing solutions. Immunohistochemistry: not tested. It is recommended that you try the antibody at 1:50-1:1,000.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Notes

Chicken products cannot be exported to Canada.

Relevant Entrez Gene Numbers: NM_020061.3

Alternate Names for Opsin 1 (Medium Wave) Antibody - BSA Free

• GCP
• GOP
• Green cone photoreceptor pigment
• Green-sensitive opsin
• medium-wave-sensitive opsin 1
• OPN1MW
• opsin 1 (cone pigments), medium-wave-sensitive 2

Background

FUNCTION: Visual pigments are the light-absorbing molecules that mediate vision. They consist of an apoprotein, opsin, covalently linked to cis-retinal. May increase spectral sensitivity in dim light. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. TISSUE SPECIFICITY: Expressed in cone photoreceptor cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Opsin 1 (Medium Wave) Antibody (NBP2-29858) (2)

Average Rating: 1

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-Fr Mouse 12/27/2016

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: mouse retinal tissues
• Species: Mouse

Comments

• Comments: Cryosection
  Primary: Chicken anti M-opsin 1:50
  Secondary: Goat anti-chicken CY3 secondary 1:100
  Procedure
  
  1.     Take the sections out from freezer and thaw the slides at room temperature for 10 minutes
  2.     Surround the tissues with a hydrophobic barrier (proper big lines!) using a barrier marker pen
  a.     Let to dry properly
  3.     Rinse the sections in 2X in PBS (5 min each)
  4.     Drain the excess wash buffer
  5.     Incubate the sections in blocking buffer (10 % NGS) for 1 h at RT
  a.     50 µl of solution
  6.     Drain the excess blocking buffer
  7.     Incubate in primary antibody overnight at 4º C in a wet chamber.
  a.     0.01 M PBS + 1 % NGS as buffer
  b.     50 µl of solution
  8.     Next day rinse the sections in PBS+triton (0.25 %) for three times (5 min each)
  9.     Incubate with Secondary antibody prepared in PBS for 2 hrs.
  a.     50 µl of solution
  10.     Rinse the sections in PBST twice (5 min each)
  11.     Rinse the sections in PBS twice (5 min each)
  12.     Drain the excess wash buffer
  a.     Air-dry for few mins
  13.     Mount with an anti-fade mounting media
  14.     Visualize using a fluorescence microscope

reviewed by: Verified Customer Immunohistochemistry-Whole Mount Mouse 12/27/2016

Summary

• Application: Other
• Sample Tested: mouse retinal tissues
• Species: Mouse

Comments

• Comments: Tested in an untested application (IHC-whole mount) and earned the Innovator's Reward for this product.
  Immunostainning of the retinal flat mount
  Prim. antibodies:      Anti M/L-cone opsin, chicken polyclonal, 1/50 dil., 1/100 dil. & 1/200 dil.
  Sec. antibodies:          Goat anti-chicken CY3, 1/100 dil.
  1.     Fix the eye for 3 hours
  2.     Dissect retina from the eye
  3.     Wash overnight in PBS
  4.     Wash the tissue in PBST 2 times; 10 min each
  a.     Triton-X 0.5 %
  5.     Block the whole mount in 10% normal goat serum (NGS) solution in PBST (1 hr)
  6.     Incubate o/n in primary antibody solution at 4 º C, primary ABs made in 1X PBST (0.05M) + 1% NGS
  7.     Next day wash in PBST + 1 % NGS; 3 X 10 min each
  8.     Incubate in secondary antibodies for 2-3 h
  a.     Protect from light with tin
  9.     Wash in PBST + 1 % NGS; 2 X 10 min each
  10.      Wash once in PBS for at least 5 min
  11.     Transfer the whole mount on the microscope slide onto a slide retina side up.
  12.     Remove PBS, add mounting medium.
  13.     Place coverslip. Observe. If correct…
  14.     Secure with nail polish
  
  Samples are C57BL retinas

Bioinformatics

• Gene Symbol: OPN1MW
• Uniprot:
  • P04000(Human)