OPA1 Antibody (1E8-1D9)

Images

 
Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.
Immunocytochemistry/ Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).
Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Immunohistochemical analysis of OPA1 on mouse skin using NBP1-71656.
Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Simple Western lane view shows a specific band for OPA1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChHaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clone
1E8-1D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

OPA1 Antibody (1E8-1D9) Summary

Immunogen
Human OPA1 [Swiss-Prot# O60313].
Localization
Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion intermembrane space
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
OPA1
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
Application Notes
This OPA1 (1E8-1D9) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Western blot, where multiple protein isoforms can be seen at approximately 90, 80 and 65 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-71656 in the following applications:

Publications
Read Publications using
NBP1-71656 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human, mouse, rat, Chinese hamster and bovine.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.1% Sodium Azide
Purity
Unpurified

Alternate Names for OPA1 Antibody (1E8-1D9)

  • BERHS
  • EC 3.6.5.5
  • FLJ12460
  • KIAA0567dynamin-like 120 kDa protein, mitochondrial
  • LargeG
  • lilr3
  • MGM1
  • mitochondrial dynamin-like GTPase
  • MTDPS14
  • NPG
  • NPGlargeG
  • NTG
  • NTGmitochondrial dynamin-like 120 kDa protein
  • OPA1
  • optic atrophy 1 (autosomal dominant)
  • Optic atrophy protein 1

Background

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Under conditions of stress, dynamin-like 120 kD protein, form S1 (which is the inactive form produced via cleavage at S1 position by OMA1) triggers the loss of mitochondrial membrane potential that results in negative regulation of the process of mitochondrial fusion. OPA1 exists as oligomeric complex consisting of membrane-bound and soluble forms of OPA1, and the latter can interacts with CHCHD3, IMMT and PARL. PARL-dependent proteolytic processing releases an antiapoptotic soluble form which is not required for mitochondrial fusion. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OPA1 Antibody (NBP1-71656)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Review for OPA1 Antibody (NBP1-71656) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-71656:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot OPA1 NBP1-71656
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reviewed by:
Anonymous
WB Human 07/25/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cell line (PC3)
SpeciesHuman

Blocking

Blocking Details5% milk in TBS buffer supplemented with 0.1% tween20 at room temperature for one hour

Primary Anitbody

Dilution Ratio1:8000

Secondary Antibody

Secondary DescriptionAnti-mouse secondary diluted at 1:10000 in 5% milk
Secondary Manufacturer Cat#SC-2005
Secondary Concentration0.4 mg/ml

Details

Detection NotesWestern blotting using ECL reagent (purchased from GE life sciences) and x-ray films.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OPA1 Antibody (NBP1-71656) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Discover related pathways, diseases and genes to OPA1 Antibody (NBP1-71656). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Blogs on OPA1.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

Understanding OPA1 and Mitochondrial Function
OPA1 belongs to the Dynamin large GTPase protein family. OPA1 exists as a single-pass membrane protein localized in the mitochondrial inner membrane and also as a soluble form in the mitochondrial intermembrane space. There, it is a key player in fusi...  Read full blog post.

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Recent Reviews

5
5
1
4
0
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0
2
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1
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Anonymous
07/25/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol OPA1
Entrez
Uniprot