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OPA1 Antibody (1E8-1D9) - BSA Free

Images

 
Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.
Immunocytochemistry/ Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.
Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChHaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clone
1E8-1D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

OPA1 Antibody (1E8-1D9) - BSA Free Summary

Immunogen
Human OPA1 [Swiss-Prot# O60313].
Localization
Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion intermembrane space
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
OPA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:25
  • Western Blot 1:1000
Application Notes
In Western blot, multiple protein isoforms can be seen at ~90, 80 and 65 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-71656 in the following applications:

Publications
Read Publications using
NBP1-71656 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for OPA1 Antibody (1E8-1D9) - BSA Free

  • BERHS
  • EC 3.6.5.5
  • FLJ12460
  • KIAA0567dynamin-like 120 kDa protein, mitochondrial
  • LargeG
  • lilr3
  • MGM1
  • mitochondrial dynamin-like GTPase
  • MTDPS14
  • NPG
  • NPGlargeG
  • NTG
  • NTGmitochondrial dynamin-like 120 kDa protein
  • OPA1
  • optic atrophy 1 (autosomal dominant)
  • Optic atrophy protein 1

Background

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Under conditions of stress, dynamin-like 120 kD protein, form S1 (which is the inactive form produced via cleavage at S1 position by OMA1) triggers the loss of mitochondrial membrane potential that results in negative regulation of the process of mitochondrial fusion. OPA1 exists as oligomeric complex consisting of membrane-bound and soluble forms of OPA1, and the latter can interacts with CHCHD3, IMMT and PARL. PARL-dependent proteolytic processing releases an antiapoptotic soluble form which is not required for mitochondrial fusion. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for OPA1 Antibody (NBP1-71656)(3)

Review for OPA1 Antibody (NBP1-71656) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-71656:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot OPA1 NBP1-71656
Enlarge
5
reviewed by:
Verified Customer
WB Human 07/25/2015
View

Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cell line (PC3)
SpeciesHuman

Blocking

Blocking Details5% milk in TBS buffer supplemented with 0.1% tween20 at room temperature for one hour

Primary Anitbody

Dilution Ratio1:8000

Secondary Antibody

Secondary DescriptionAnti-mouse secondary diluted at 1:10000 in 5% milk
Secondary Manufacturer Cat#SC-2005
Secondary Concentration0.4 mg/ml

Details

Detection NotesWestern blotting using ECL reagent (purchased from GE life sciences) and x-ray films.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OPA1 Antibody (NBP1-71656) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for OPA1 Antibody (NBP1-71656)

Discover related pathways, diseases and genes to OPA1 Antibody (NBP1-71656). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for OPA1 Antibody (NBP1-71656)

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Blogs on OPA1.

Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.

BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.

Understanding OPA1 and Mitochondrial Function
OPA1 belongs to the Dynamin large GTPase protein family. OPA1 exists as a single-pass membrane protein localized in the mitochondrial inner membrane and also as a soluble form in the mitochondrial intermembrane space. There, it is a key player in fusi...  Read full blog post.

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Verified Customer
07/25/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol OPA1
Entrez
Uniprot