OPA1 Antibody (1E8-1D9)

Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.

Immunocytochemistry/Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with Dapi (blue) and Phalloidin (red).

Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Immunohistochemical analysis of OPA1 on mouse skin using NBP1-71656.

Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Simple Western lane view shows a specific band for OPA1 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt, Bv, ChHa
• Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
• Clone: 1E8-1D9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

OPA1 Antibody (1E8-1D9) Summary

• Immunogen: Human OPA1 [Swiss-Prot# O60313].
• Localization: Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion intermembrane space
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: OPA1
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
  • Simple Western 1:25
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
• Application Notes: This OPA1 (1E8-1D9) antibody is useful for Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and Western blot, where multiple protein isoforms can be seen at approximately 90, 80 and 65 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Reactivity Notes

Human, mouse, rat, Chinese hamster and bovine.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.1% Sodium Azide
• Purity: Unpurified

Alternate Names for OPA1 Antibody (1E8-1D9)

• BERHS
• EC 3.6.5.5
• FLJ12460
• KIAA0567dynamin-like 120 kDa protein, mitochondrial
• LargeG
• lilr3
• MGM1
• mitochondrial dynamin-like GTPase
• MTDPS14
• NPG
• NPGlargeG
• NTG
• NTGmitochondrial dynamin-like 120 kDa protein
• OPA1
• optic atrophy 1 (autosomal dominant)
• Optic atrophy protein 1

Background

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Under conditions of stress, dynamin-like 120 kD protein, form S1 (which is the inactive form produced via cleavage at S1 position by OMA1) triggers the loss of mitochondrial membrane potential that results in negative regulation of the process of mitochondrial fusion. OPA1 exists as oligomeric complex consisting of membrane-bound and soluble forms of OPA1, and the latter can interacts with CHCHD3, IMMT and PARL. PARL-dependent proteolytic processing releases an antiapoptotic soluble form which is not required for mitochondrial fusion. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for OPA1 Antibody (NBP1-71656)(2)

Publications using NBP1-71656

• Bollu LR, Ren J, Blessing AM et al. Involvement of de novo synthesized palmitate and mitochondrial EGFR in EGF induced mitochondrial fusion of cancer cells. Cell Cycle 2014 [PMID: 25483192]
• Montaigne D, Marechal X, Coisne A et al. Myocardial Contractile Dysfunction is Associated with Impaired Mitochondrial Function and Dynamics in Type 2 Diabetic but not in Obese Patients. Circulation. 2014 Jun 13 [PMID: 24928681] (WB, Human)

Review for OPA1 Antibody (NBP1-71656) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Anonymous WB Human 07/25/2015

Summary

• Application: Western Blot
• Sample Tested: Human prostate cancer cell line (PC3)
• Species: Human

Blocking

• Blocking Details: 5% milk in TBS buffer supplemented with 0.1% tween20 at room temperature for one hour

Primary Anitbody

• Dilution Ratio: 1:8000

Secondary Antibody

• Secondary Description: Anti-mouse secondary diluted at 1:10000 in 5% milk
• Secondary Manufacturer Cat#: SC-2005
• Secondary Concentration: 0.4 mg/ml

Details

• Detection Notes: Western blotting using ECL reagent (purchased from GE life sciences) and x-ray films.

Bioinformatics

• Gene Symbol: OPA1
• Entrez:
  • 4976(Human)
  • 74143(Mouse)
  • 171116(Rat)
• Uniprot:
  • O60313()
  • P58281(Mouse)
  • Q2TA68(Rat)