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Home » OPA1 » OPA1 Antibodies » OPA1 Antibody (1E8-1D9) - BSA Free

OPA1 Antibody (1E8-1D9) - BSA Free

 (3 Publications)
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Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.
Western Blot: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 expression in 1) HeLa 2) MEF 3) HepG2 4) A431 5) CHO 6)PC12 and 7) Ntera2 whole cell lysates using NBP1-71656.
Immunocytochemistry/ Immunofluorescence: OPA1 Antibody (1E8-1D9) [NBP1-71656] - OPA1 antibody was tested in ARPE-19 cells with FITC (green). Nuclei and actin were counterstained with DAPI (blue) and Phalloidin (red).
Immunohistochemistry: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Analysis of OPA1 on mouse skin using NBP1-71656.
Simple Western: OPA1 Antibody (1E8-1D9) [NBP1-71656] - Image shows a specific band for OPA1 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, ChHaSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clone
1E8-1D9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-71656AF350
Alexa Fluor 405
NBP1-71656AF405
Alexa Fluor 488
NBP1-71656AF488
Alexa Fluor 532
NBP1-71656AF532
Alexa Fluor 594
NBP1-71656AF594
Alexa Fluor 647
NBP1-71656AF647
Alexa Fluor 700
NBP1-71656AF700
Alexa Fluor 750
NBP1-71656AF750
Biotin
NBP1-71656B
DyLight 350
NBP1-71656UV
DyLight 405
NBP1-71656V
DyLight 488
NBP1-71656G
DyLight 550
NBP1-71656R
DyLight 594
NBP1-71656DL594
DyLight 650
NBP1-71656C
DyLight 680
NBP1-71656FR
DyLight 755
NBP1-71656IR
FITC
NBP1-71656F
HRP
NBP1-71656H
Janelia Fluor 549
NBP1-71656JF549
Janelia Fluor 646
NBP1-71656JF646
View Available Formulations
Catalog# & Formulation Size Price
BSA Free
NBP1-71656SS
100% Guarantee on every product

OPA1 Antibody (1E8-1D9) - BSA Free Summary

Immunogen
Human OPA1 [Swiss-Prot# O60313].
Localization
Mitochondrion inner membrane; Single-pass membrane protein. Mitochondrion intermembrane space
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
OPA1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:50
  • Immunohistochemistry 1:100
  • Immunohistochemistry-Paraffin 1:100
  • Simple Western 1:25
  • Western Blot 1:1000
Application Notes
In Western blot, multiple protein isoforms can be seen at ~90, 80 and 65 kDa.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-71656 in the following applications:
Publications
Read Publications using
NBP1-71656 in the following applications:

  • IHC
    1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for OPA1 Antibody (1E8-1D9) - BSA Free

  • BERHS
  • EC 3.6.5.5
  • FLJ12460
  • KIAA0567dynamin-like 120 kDa protein, mitochondrial
  • LargeG
  • lilr3
  • MGM1
  • mitochondrial dynamin-like GTPase
  • MTDPS14
  • NPG
  • NPGlargeG
  • NTG
  • NTGmitochondrial dynamin-like 120 kDa protein
  • OPA1
  • optic atrophy 1 (autosomal dominant)
  • Optic atrophy protein 1

Background

OPA1 (optic atrophy protein 1) is a dynamin-related GTPase which forms a diffusion barrier for proteins stored in mitochondrial cristae and is critical to mitochondrial fusion as well as regulation of apoptosis. Intrinsic apoptotic signals mediated proteolytic processing leads OPA1 oligomers disassembly and caspase activator cytochrome C (CYCS) release into mitochondrial intermembrane spaces. Under conditions of stress, dynamin-like 120 kD protein, form S1 (which is the inactive form produced via cleavage at S1 position by OMA1) triggers the loss of mitochondrial membrane potential that results in negative regulation of the process of mitochondrial fusion. OPA1 exists as oligomeric complex consisting of membrane-bound and soluble forms of OPA1, and the latter can interacts with CHCHD3, IMMT and PARL. PARL-dependent proteolytic processing releases an antiapoptotic soluble form which is not required for mitochondrial fusion. Defects in OPA1 are a cause of optic atrophy type 1 (OPA1) as well as dominant optic atrophy plus syndrome (DOA+)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-55288
Flow Cytometry: DRP1 Antibody [NB110-55288] - An intracellular stain was performed on HeLa cells with NB110-55288 and a matched isotype control. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by Rabbit IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550.
Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton X-100. The cells were incubated with anti-DRP1 at 5 ug/mL overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunocytochemistry/Immunofluorescence: DRP1 Antibody [NB110-55288] - NIH3T3 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DRP1 Antibody at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
(3 Reviews)
(35 Publications)
Species: Fi, Hu, Mu, Pm, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
Mitofusin 2
Antibody - BSA Free
NBP2-66383
Immunohistochemistry-Paraffin: Mitofusin 2 Antibody [NBP2-66383] - Analysis of FFPE tissue section of mouse heart using Mitofusin 2/MFN2 antibody at 5 ug/mL concentration (1:200 dilution). The primary antibody binding to MFN2 antigen was detected using HRP conjugated anti-rabbit secondary antibody with DAB reagent, and the sections were further counterstained with hematoxylin for labeling cellular nuclei. This MFN2 antibody generated a diffused to punctate cytoplasmic staining of mitochondria in the cardiac muscles (brown color). The nuclei of the muscle cells and the macrophages are stained blue in this image (40X image of a transverse section of heart tissue).
Immunocytochemistry/Immunofluorescence: Mitofusin 2 Antibody [NBP2-66383] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-Mitofusin 2 at 5 ug/mL overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at 1:500. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at 1:1000 and detected with an anti-mouse DyLight 550 (Red) at 1:500. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: Mitofusin 2 Antibody [NBP2-66383] - Total protein from human HeLa and A431, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 0.5 ug/mL anti-MFN2 in block buffer and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Mitofusin 1
Antibody (3C9)
H00055669-M04
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - MFN1 monoclonal antibody (M04), clone 3C9 Western Blot analysis of MFN1 expression in HeLa ( Cat # L013V1 ).
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Western blot analysis of MFN1 over-expressed 293 cell line, cotransfected with MFN1 Validated Chimera RNAi or non-transfected control. Blot probed with H00055669-M04. GAPDH (36.1 kDa) used as loading control.
Western Blot: Mitofusin 1 Antibody (3C9) [H00055669-M04] - Analysis of MFN1 expression in transfected 293T cell line by MFN1 monoclonal antibody (M04), clone 3C9. Lane 1: MFN1 transfected lysatE (84.2 KDa). Lane 2: Non-transfected lysate.
(26 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IP, KD, WB
Utrophin Antibody
NBP1-84074
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human prostate shows moderate to strong membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human kidney shows moderate membranous positivity in cells in glomeruli and cells in tubules.
Immunohistochemistry-Paraffin: Utrophin Antibody [NBP1-84074] - Staining of human stomach shows moderate membranous positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P
DENR Antibody
NBP2-13913
Western Blot: DENR Antibody [NBP2-13913] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells)Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
Immunohistochemistry-Paraffin: DENR Antibody [NBP2-13913] - Staining of human testis shows moderate cytoplasmic and nuclear positivity in cells in seminiferus ducts.
Western Blot: DENR Antibody [NBP2-13913] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-159
(1 Publication)
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
DAP Kinase 2
Antibody (OTI1C5)
NBP2-02477
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Analysis of Jurkat cells, using anti-DAP Kinase 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-DAP Kinase 2 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) [NBP2-02477] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
CRMP1 Antibody
(JU73-30)
NBP2-75449
Flow Cytometry: CRMP1 Antibody (JU73-30) [NBP2-75449] - Analysis of SH-SY-5Y cells with CRMP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
Immunocytochemistry/Immunofluorescence: CRMP1 Antibody (JU73-30) [NBP2-75449] - Staining CRMP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Immunocytochemistry/Immunofluorescence: CRMP1 Antibody (JU73-30) [NBP2-75449] - Staining CRMP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
Cytochrome c
Antibody (7H8.2C12) - ...
NB100-56503
Flow Cytometry: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Using the Alexa Fluor 488 direct conjugate, an intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503AF488 (blue) and a matched isotype control NBP2-27231AF488 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): Cytochrome c Antibody (7H8.2C12) [NB100-56503] - An intracellular stain was performed on HeLa cells with Cytochrome c (7H8.2C12) antibody NB100-56503PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
Western Blot: Cytochrome c Antibody (7H8.2C12) [NB100-56503] - Metformin activates apoptotic cell death; Right panel: MCF-7 cells were kept in the presence or absence of metformin for 20 days and then processed to obtain mitochondrial or whole cellular extracts. BAX and CYCS expression levels were determined by Western blot as indicated under Material and Methods. Densitometric analysis of the gels was performed as indicated under Material and Methods. PHB and CDK4 were used as purity and loading controls. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/2073-4409/8/1/49), licensed under a CC-BY licence.
(58 Publications)
Species: Dr, Hu, Mu, Rb, Rt
Applications: Flow-CS, Flow-IC, Flow, IB, ICC/IF, IHC, IHC-P, Simple Western, WB
TTC11 Antibody
NB100-56646
Western Blot: TTC11 Antibody [NB100-56646] - Effect of liraglutide on brain cortical mitochondrial fission/fusion markers in female mice with early AD-like pathology. Brain cortical Fis1 (A) protein levels were determined and normalized to beta-actin levels, and representative Western blotting images displayed. Data are the mean +/- SE from 6 mice/group. Statistical significance: * p < 0.05, by the one-way ANOVA with the Fisher LSD post-hoc test for multiple comparisons. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/1422-0067/21/5/1746), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: TTC11 Antibody [NB100-56646] - Analysis of methcarn-fixed, paraffin-embedded hippocampus from control (normal) and Alzheimer brain. Quantification in five AD brains and five age-matched controls indicated that Fis1 was detected in only the soma in ~80% of pyramidal neurons in AD hippocampus, significantly different from what was seen for control hippocampal neurons. Source: Wang et al, 2009.
Knockdown Validated: TTC11 Antibody [NB100-56646] - Analysis of Fis1 in A) human HeLa and B) mouse NIH 3T3 lysate using Fis1 antibody at 1:1000 dilution. B. Specificity validation of the Fis1 antibody. Fis1 was analyzed in M17 cells stably transfected with vector only, Fis1 RNAi, Fis1 (Fis1wt OE) or left untransfected (negative control). The western blot data showed that M17 cells expressed Fis1 (Vector, Untransfected control) which could be knocked down by Fis1 RNAi. Cells transfected with Fis1 overexpressed Fis1. M17 is a human neuroblastoma cell line.
(27 Publications)
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KD, WB
Substance P
Antibody (266815)
MAB4375
Substance P was detected in immersion fixed paraffin-embedded sections of human spinal cord using 8 µg/mL Human/Mouse/Rat Substance P Monoclonal Antibody (Catalog # MAB4375) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to the spinal cord dorsal horn. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
(3 Reviews)
(5 Publications)
Species: Hu, Mu, Rt
Applications: IHC
PARL Antibody
AF7306
Western blot shows lysates of DU145 human prostate carcinoma cell line, MCF‑7 human breast cancer cell line, and mouse brown adipose tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human/Mouse/Rat PARL Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7306) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for PARL at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Species: Hu, Mu, Rt
Applications: WB
OPA3 Antibody
(OTI3G4)
NBP2-46294
Immunohistochemistry-Paraffin: OPA3 Antibody (OTI3G4) [NBP2-46294] - Staining of paraffin-embedded Human prostate tissue within the normal limits using anti-OPA3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min, (1:150).
Immunohistochemistry-Paraffin: OPA3 Antibody (OTI3G4) [NBP2-46294] - Staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-OPA3 mouse monoclonal antibody. (Heatinduced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min) (1:150).
Immunohistochemistry-Paraffin: OPA3 Antibody (OTI3G4) [NBP2-46294] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-OPA3 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 degrees C for 3 min)(1:150)
Species: Hu
Applications: IHC, IHC-P, WB
DNA Polymerase
gamma Antibody
NBP1-52300
Immunohistochemistry-Paraffin: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with human skeletal muscle at concentration 10 ug/ml.
Western Blot: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with (30 ug of whole cell lysate). A: 293T. 5% SDS PAGE. POLG antibody diluted at 1:5000.
Immunocytochemistry/Immunofluorescence: DNA Polymerase gamma Antibody [NBP1-52300] - Analysis of anti-POLG antibody with methanol-fixed HeLa cell at 1:200 dilution.
(4 Publications)
Species: Hu, Mu
Applications: ICC, ICC/IF, IHC, IHC-P, WB
Bax Antibody (6A7)
NBP1-28566
Western Blot: Bax Antibody (6A7) [NBP1-28566] - Analysis of lysates from human metastatic pancreatic cancer cell line L3.6pl using Bax Antibody (clone 6A7; Lot # B168-P780E). This image was submitted via reviews by a varified end user.
Western Blot: Bax Antibody (6A7) [NBP1-28566] - rOPN administration elevated the expression of autophagy-related proteins while suppressing apoptosis in rat brain at 24 h after SAH. The effects of rOPN on expression levels of Bax, mean +/- SD is 1.006 +/- 0.321 in Sham group, 37.47 +/- 10.86 in SAH + Vehicle group, 23.83 +/- 8.143 in SAH + rOPN group, F = 33.13, in the left hemisphere of rat brain at 24 h after SAH. Sample size is 18, n = 6 per group. Data were presented as mean +/- SD. *P < .05, ***P < .001 vs Sham group; #P < .05, ##P < .01 vs SAH + Vehicle group Image collected and cropped by CiteAb from the following publication (https://onlinelibrary.wiley.com/doi/abs/10.1111/cns.13199) licensed under a CC-BY licence.
Immunohistochemistry-Frozen: Bax Antibody (6A7) [NBP1-28566] - Mouse retina with no pretreatment. IHC-F image submitted by a verified customer review
(3 Reviews)
(17 Publications)
Species: Bv, Hu, Pm, Mu, Po, Rt
Applications: Func, IHC, IHC-Fr, IHC-P, WB
SPG7 Antibody
(OTI1C1)
NBP2-01860
Western Blot: SPG7 Antibody (OTI1C1) [NBP2-01860] - Loss of AFG3L2 and/or YME1L is associated with elevated OMA1, diminished SPG7, and a markedly altered pattern of OPA1 protein forms. Whole-cell lysates were resolved using SDS-PAGE and western blotted with antibody to Drp1, Oma1, and Spg7. Image collected and cropped by CiteAb from the following publication (http://www.mdpi.com/1422-0067/19/12/3930), licensed under a CC-BY licence.
Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Hela cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
Flow Cytometry: SPG7 Antibody (OTI1C1) [NBP2-01860] - Analysis of Jurkat cells, using anti-SPG7 antibody, (Red), compared to a nonspecific negative control antibody (Blue).
(1 Review)
(3 Publications)
Species: Ca, Hu, Pm, Mu, Rt
Applications: Flow, ICC/IF, IHC, WB
Prohibitin
Antibody [Unconjugate
AF3470
Prohibitin was detected in immersion fixed MCF‑7 human breast cancer cell line using Goat Anti-Human Prohibitin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3470) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Species: Hu
Applications: ICC, WB
p53 Antibody (PAb
240) - BSA Free
NB200-103
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Flow Cytometry: p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on A549 cells with p53 (PAb240) NB200-103AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
Flow (Intracellular): p53 Antibody (PAb 240) [NB200-103] - An intracellular stain was performed on HeLa cells with p53 (PAb240) NB200-103 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature, followed by APC-conjugated anti-mouse IgG secondary antibody F0101B.
(3 Reviews)
(33 Publications)
Species: Hu, Mu, Rt, Xp(-), Ye
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
OPA1 Antibody
(1E8-1D9) - BSA Free
NBP1-71656
OPA1 Antibody (1E8-1D9) - BSA Free
Species: Hu, Mu, Rt, Bv, ChHa
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P

Publications for OPA1 Antibody (NBP1-71656)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, WB.


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Review for OPA1 Antibody (NBP1-71656) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-71656:
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Images Ratings Applications Species Date Details
Western Blot OPA1 NBP1-71656
Enlarge 		5
reviewed by:
Verified Customer
 WB Human 07/25/2015
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Summary

ApplicationWestern Blot
Sample TestedHuman prostate cancer cell line (PC3)
SpeciesHuman

Blocking

Blocking Details5% milk in TBS buffer supplemented with 0.1% tween20 at room temperature for one hour

Primary Anitbody

Dilution Ratio1:8000

Secondary Antibody

Secondary DescriptionAnti-mouse secondary diluted at 1:10000 in 5% milk
Secondary Manufacturer Cat#SC-2005
Secondary Concentration0.4 mg/ml

Details

Detection NotesWestern blotting using ECL reagent (purchased from GE life sciences) and x-ray films.

Product General Protocols

View specific protocols for OPA1 Antibody (NBP1-71656): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for OPA1 Antibody (NBP1-71656) (0)

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Secondary Antibodies

 

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Blogs on OPA1.

Check out the latest blog posts on OPA1.
Losing memory: Toxicity from mutant APP and amyloid beta explain the hippocampal neuronal damage in Alzheimer's disease
 By Jamshed Arslan Pharm.D.  Alzheimer's disease (AD) is an irreversible brain disorder that destroys memory and thinking skills. The telltale signs of AD brains are extracellular deposits of amy...  Read full blog post.
BNIP3 - a regulator of mitochondrial autophagy and cell death
Bcl-2 nineteen-kilodalton interacting protein 3 (BNIP3) is a pro-apoptotic BH3-only protein. BNIP3 localizes to the mitochondrial membrane where it plays a key role in mitochondrial autophagy and cell death pathways. Similar to other Bcl-2 family m...  Read full blog post.
Understanding OPA1 and Mitochondrial Function
OPA1 belongs to the Dynamin large GTPase protein family. OPA1 exists as a single-pass membrane protein localized in the mitochondrial inner membrane and also as a soluble form in the mitochondrial intermembrane space. There, it is a key player in fusi...  Read full blog post.
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Western Blot OPA1 NBP1-71656
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 Verified Customer
07/25/2015
Application: WB
Species: Human
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Bioinformatics

Gene Symbol OPA1
Entrez
Uniprot