Oncostatin M/OSM Antibody (OTI2B6)

Western Blot: Oncostatin M/OSM Antibody (2B6) [NBP1-47904] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Oncostatin M (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Oncostatin M.

Immunohistochemistry-Paraffin: Oncostatin M/OSM Antibody (2B6) [NBP1-47904] - Staining of paraffin-embedded lung tissue using anti-Oncostatin M mouse monoclonal antibody.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI2B6
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

Oncostatin M/OSM Antibody (OTI2B6) Summary

• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 25-252 of human OSM
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: OSM
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:1000
• Theoretical MW: 25.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Oncostatin M/OSM Antibody (OTI2B6)

• MGC20461
• oncostatin M
• oncostatin-M
• OSM

Background

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Oncostatin M/OSM Antibody (NBP1-47904)(2)

Publications using NBP1-47904

• Liu Q, Lan T, Song Y et al. Oncostatin M expression and TP53 mutation status regulate tumor-infiltration of immune cells and survival outcomes in cholangiocarcinoma Onco Targets Ther 2020-11-10 [PMID: 33216732] (IF/IHC, Human)
• Kang Mee Joo, Kim Jayoun, Jang Jin-Young et al. 22q11-q13 as a hot spot for prediction of disease-free survival in bile duct cancer: integrative analysis of copy number variations. Cancer Genet. 2014-02-15 [PMID: 24703811] (Human)

Bioinformatics

• Gene Symbol: OSM
• Entrez:
  • 5008(Human)
• Uniprot:
  • P13725()