OLFM-L3 Antibody

Western Blot: OLFM-L3 Antibody [NBP2-15055] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with OLFML3 antibody [N1C1] diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: OLFM-L3 Antibody [NBP2-15055] - Analysis of methanol-fixed A549, using Olfactomedin-like 3 antibody at 1:500 dilution.

Immunohistochemistry-Paraffin: OLFM-L3 Antibody [NBP2-15055] - Mouse heart. Olfactomedin-like 3 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.

• Reactivity: Hu, Mu, Rt, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

OLFM-L3 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human OLFML3. The exact sequence is proprietary.
• Predicted Species: Rat (93%), Rhesus Macaque (99%), Bovine (96%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OLFML3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 46 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for OLFM-L3 Antibody

• HNOEL-iso
• HNOEL-isoOLF44
• hOLF44
• OLF44
• olfactomedin-like 3
• olfactomedin-like protein 3
• OLFML3
• OLFM-L3

Background

Secreted scaffold protein that plays an essential role in dorsoventral patterning during early development. Stabilizes axial formation by restricting chordin (CHRD) activity on the dorsal side. Acts by facilitating the association between the tolloid proteases and their substrate chordin (CHRD), leading to enhance chordin (CHRD) degradation (By similarity). May have matrix-related function involved in placental and embryonic development, or play a similar role in other physiological processes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Bioinformatics

• Gene Symbol: OLFML3
• Uniprot:
  • Q9NRN5(Human)