MAF1 Antibody

Western Blot: MAF1 Antibody [NBP1-31617] - Wild-type (WT) and MAF1 knockout (KO) 293T cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody

Immunocytochemistry/Immunofluorescence: MAF1 Antibody [NBP1-31617] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.

Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617] - Human colon carcinoma. MAF1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: MAF1 Antibody [NBP1-31617] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with MAF1 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617] - Human endometrial carcinoma. MAF1 antibody dilution: 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

• Reactivity: Hu, Mu, Bv, RM
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MAF1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the center region of human MAF1. The exact sequence is proprietary.
• Localization: Nucleus, Cytoplasm
• Predicted Species: Rhesus Macaque (98%), Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MAF1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 29 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (86%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7).
• Preservative: 0.01% Thimerosal
• Purity: Immunogen affinity purified

Alternate Names for MAF1 Antibody

• DKFZp586G1123
• homolog of yeast MAF1
• MAF1 homolog (S. cerevisiae)
• MGC20332
• MGC31779
• MGC39758
• repressor of RNA polymerase III transcription MAF1 homolog

Background

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for MAF1 Antibody (NBP1-31617)(1)

Publication using NBP1-31617

• Tsang CK, Mi Q, Su G et al. Maf1 is an intrinsic suppressor against spontaneous neural repair and functional recovery after ischemic stroke Journal of advanced research 2022-11-17 [PMID: 36402285] (WB, IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: MAF1