MAF1 Antibody

Images

 
Western Blot: MAF1 Antibody [NBP1-31617] - WB analysis of 30 ug whole cell lysate of GL261 cells (A), BCL-1 cells (B) and Raw264.7 cells (C) on 12 % SDS-PAGE using MAF1 antibody at 1:1000 dilution
Immunocytochemistry/ Immunofluorescence: MAF1 Antibody [NBP1-31617] - Paraformaldehyde-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617] - IHC-P analysis of paraffin-embedded human colon carcinoma using 1:500 dilution of MAF1 antibody.
Western Blot: MAF1 Antibody [NBP1-31617] - Sample(30 ug whole cell lysate)A:A431 B:MOLT4 12% SDS PAGE, antibody diluted at 1:1000.
Western Blot: MAF1 Antibody [NBP1-31617] - WB analysis of 30 ug PC-12 whole cell lysate on 12 % SDS-PAGE using MAF1 antibody at 1:1000 dilution
Immunohistochemistry-Paraffin: MAF1 Antibody [NBP1-31617] - IHC-P analysis of paraffin-embedded human endometrial carcinoma using 1:500 dilution of MAF1 antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, RMSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

MAF1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human MAF1. The exact sequence is proprietary.
Localization
Nucleus, Cytoplasm
Predicted Species
Rhesus Macaque (98%), Bovine (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAF1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
29 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (86%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MAF1 Antibody

  • DKFZp586G1123
  • homolog of yeast MAF1
  • MAF1 homolog (S. cerevisiae)
  • MGC20332
  • MGC31779
  • MGC39758
  • repressor of RNA polymerase III transcription MAF1 homolog

Background

This gene encodes a protein that is similar to Maf1, a Saccharomyces cerevisiae protein highly conserved in eukaryotic cells. Yeast Maf1 is a negative effector of RNA polymerase III (Pol III). It responds to changes in the cellular environment and represses pol III transcription. Biochemical studies identified the initiation factor TFIIIB as a target for Maf1-dependent repression. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MAF1 Antibody (NBP1-31617) (0)

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Secondary Antibodies

 

Isotype Controls

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Calculators

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Bioinformatics

Gene Symbol MAF1