OGG1 Antibody (2B4) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - A431 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with OGG1 (2B4) at a 1:100 dilution overnight at 4 degrees Celsius and detected with DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were detected with DAPI (Blue) at 2.0 ug/ml in 1X PBS. Cells were imaged using a 40X objective. Image from the standard format of this antibody.

Immunohistochemistry: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells rectum adenocarcinoma. Image from the standard format of this antibody.

Immunohistochemistry: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells endometrial carcinoma. Image from the standard format of this antibody.

Immunohistochemistry: OGG1 Antibody (2B4) - Azide and BSA Free [NBP2-80891] - Analysis of OGG1 antibody (clone 4E8) at 5 ug/ml generated a general cytoplasmic staining pattern with intense nuclear staining in select cells renal cell carcinoma. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: Flow, ICC/IF, IHC, CyTOF-ready
• Clone: 2B4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.9 mg/ml

OGG1 Antibody (2B4) - Azide and BSA Free Summary

• Immunogen: Partial recombinant protein made to an internal sequence of the human OGG1 protein (between amino acids 100-300) [Uniprot: O15527]
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: OGG1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry 2-5 ug/ml
  • Immunocytochemistry/ Immunofluorescence 10 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 5 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 0.9 mg/ml
• Purity: Protein A or G purified

Alternate Names for OGG1 Antibody (2B4) - Azide and BSA Free

• 8-hydroxyguanine DNA glycosylase
• 8-oxoguanine DNA glycosylase
• AP lyase
• DNA-apurinic or apyrimidinic site lyase
• HOGG1
• MMH
• MUTMOGH1HMMH
• N-glycosylase/DNA lyase
• OGG1

Background

OGG1, also known as 8-oxoguanine glycosylase, is a DNA glycosylase enzyme involved in base excision repair. This protein is the primary enzyme responsible for the excision of 7,8-dihydro-8-oxoguanine (8-oxoG), a mutagenic base byproduct which occurs as a result of exposure to reactive oxygen species (ROS). It has a beta lyase activity that nicks DNA 3' to the lesion. Recombinant human OGG1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: OGG1