Occludin Antibody - BSA Free

Western Blot: Occludin Antibody [NBP1-77037] - Western blot analysis of OCLN in human liver tissue lysate with OCLN antibody at 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: Occludin Antibody - BSA Free [NBP1-77037] - Immunofluorescence of Occludin in mouse brain tissue with Occludin antibody at 20 ug/mL.Red: Occludin Antibody Blue: DAPI staining

Immunohistochemistry: Occludin Antibody - BSA Free [NBP1-77037] - Immunohistochemistry of Occludin in mouse brain tissue with Occludin Antibody at 5 ug/mL.

Immunohistochemistry: Occludin Antibody - BSA Free [NBP1-77037] - Immunohistochemistry of Occludin in human liver tissue with Occludin antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: Occludin Antibody - BSA Free [NBP1-77037] - Immunofluorescence of Occludin in Human Liver tissue with Occludin antibody at 20 u/mL.

Immunocytochemistry/ Immunofluorescence: Occludin Antibody - BSA Free [NBP1-77037] - Immunofluorescence of Occludin in human liver tissue with Occludin antibody at 20 ug/ml.Red: Occludin Antibody Blue: DAPI staining

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Occludin Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human OCLN. The immunogen is located within the last 50 amino acids of OCLN. Amino Acid Squence: SHIKKMVGDYDRQKT
• Marker: Tight Junctions Marker
• Specificity: At least three isoforms of OCLN are known to exist.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: OCLN
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/mL
  • Immunohistochemistry 2.5 ug/ml
  • Immunohistochemistry-Paraffin 2.5 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  Occludin Peptide (NBP1-77037PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for Occludin Antibody - BSA Free

• BLCPMG
• Occludin
• OCLN

Background

Tight junctions act as a semi-permeable barrier to the transport of ions, solutes, and water and are considered to function as a fence that divides apical and basolateral domains of plasma membranes. Tight junctions coordinate a variety of signaling and trafficking molecules regulating cell differentiation, proliferation, and polarity and contain a number of junctional proteins including Occludin, Claudins, junctional adhesion molecules (JAMs), as well as multiple scaffold proteins. Occludin, the first identified component of tight junction strands, is thought function as a signal transmitter in multiple signaling pathways and can associate with multiple kinases and phosphatases such as PI3-kinase and protein phosphatases 1 and 2A. At least two isoforms of OCLN are known to exist.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Occludin Antibody (NBP1-77037)(4)

Publications using NBP1-77037

• Yuan J, Zhang Y, Zhang Y et al. Effects of metal nanoparticles on tight junction-associated proteins via HIF-1alpha/miR-29b/MMPs pathway in human epidermal keratinocytes Particle and fibre toxicology 2021-03-19 [PMID: 33740985]
• Lee PC, Hsieh YC, Huo TI et al. Active Vitamin D3 Treatment Attenuated Bacterial Translocation via Improving Intestinal Barriers in Cirrhotic Rats Molecular nutrition & food research 2020-11-30 [PMID: 33258263] (WB, Rat)
• Horn La, Riskin J, Hempel Ha et Al. Simultaneous inhibition of CXCR1/2, TGF-beta, and PD-L1 remodels the tumor and its microenvironment to drive antitumor immunity J Immunother Cancer 2020-03-01 [PMID: 32188703] (IF, Human, Mouse)
• Liu Q, Xu C, Ji G et al. Sublethal effects of zinc oxide nanoparticles on male reproductive cells. Toxicol In Vitro 2016-05-28 [PMID: 27247145] (ICC/IF, WB)

Bioinformatics

• Gene Symbol: OCLN
• Entrez:
  • 100506658(Human)
  • 100506658(Human)
  • 83497(Rat)
• Uniprot:
  • Q16625()