NUP133 Antibody

Western Blot:[NBP3-29044]-Detection of human NUP133 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa and HEK293T cells. Antibodies: Affinity purified rabbit anti-NUP133 antibody used for WB at 0. 04 µg/ml and 1 µg/ml and used for IP at 10 µg/mg lysate. NUP133 was also immunoprecipitated by rabbit anti-NUP133 antibody , which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes and 10 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NUP133 Antibody Summary

• Description: Novus Biologicals Rabbit NUP133 Antibody (NBP3-29044) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: between 1106 and 1156
• Assay Type: [Assay Type]
• Sample Volume: [Sample Volume]
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUP133
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-buffered Saline 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Affinity purified

Alternate Names for NUP133 Antibody

• FLJ10814
• hNUP133,133 kDa nucleoporin
• MGC21133
• nuclear pore complex protein Nup133
• nucleoporin 133kD
• nucleoporin 133kDa
• Nucleoporin Nup133

Background

The nuclear envelope creates distinct nuclear and cytoplasmic compartments in eukaryotic cells. It consists of two concentric membranes perforated by nuclear pores, large protein complexes that form aqueous channels to regulate the flow of macromolecules between the nucleus and the cytoplasm. These complexes are composed of at least 100 different polypeptide subunits, many of which belong to the nucleoporin family. The nucleoporin protein encoded by this gene displays evolutionarily conserved interactions with other nucleoporins. This protein, which localizes to both sides of the nuclear pore complex at interphase, remains associated with the complex during mitosis and is targeted at early stages to the reforming nuclear envelope. This protein also localizes to kinetochores of mitotic cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUP133