Numb Antibody

Product Discontinued

Numb Antibody Summary

• Immunogen: Antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human NUMB. The immunogen is located within the last 50 amino acids of NUMB.
• Specificity: At least four isoforms of NUMB are known to exist; this antibody will detect all of them.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NUMB
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 5 ug/ml
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Numb Antibody

• h-Numb
• mnb
• m-numb
• numb (Drosophila) homolog
• numb homolog (Drosophila)
• Numb
• protein numb homolog
• Protein S171
• S171

Background

NUMB, the mammalian homolog to the Drosophila asymmetric cell fate determinant NUMB, is thought to share several features molecular mechanisms in mammalian cells, generating asymmetric cell divisions during neurogenesis in vertebrate development as well as in hematopoietic stem cells. NUMB has been shown to inhibit Notch signaling, and is itself regulated by ubiquitinylation by MDM2. NUMB has also been shown to help activate the tumor suppressor p53, suggesting that loss of NUMB in cancerous cells would not only activate the potential oncogene Notch, but diminish the tumor suppressing effect of p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUMB