| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-21404-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Azide and BSA Free | NBP2-99081-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-55985-25ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-26452-50ul | |||
![Western Blot: Nuf2 Antibody [NBP1-31632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP1-31632-img0009.jpg "Western Blot: Nuf2 Antibody [NBP1-31632] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.")
![Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - NUF2 antibody detects NUF2 protein at kinetochore by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: NUF2 stained by NUF2 antibody diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:500. Blue: Fluoroshield with DAPI.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0008.jpg)
![Western Blot: Nuf2 Antibody [NBP1-31632] - HeLa whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NUF2 antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP1-31632-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - A431 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: CDCA1 protein stained by CDCA1 antibody [312] diluted at 1:200. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP1-31632] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using CDCA1antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-31632-img0006.jpg)
![Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG](http://images.novusbio.com/fullsize/nbp3-21404_rabbit-nuf2-pab-45202315493220.jpg "Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT4 | Lane 3: U-251 MG")
![Western Blot: Nuf2 Antibody (312) [NBP2-43780] - Analysis of whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDCA1 antibody [312] at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Nuf2-Antibody-312-Western-Blot-NBP2-43780-img0001.jpg "Western Blot: Nuf2 Antibody (312) [NBP2-43780] - Analysis of whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDCA1 antibody [312] at a dilution of 1:1000.")
![Immunoprecipitation: Nuf2 Antibody (312) [NBP2-43780] - Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 ug of NUF2 antibody [312]. Western blot analysis was performed using NUF2 antibody [312]. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Nuf2-Antibody-312-Immunoprecipitation-NBP2-43780-img0005.jpg)
![Immunohistochemistry-Paraffin: Nuf2 Antibody (312) [NBP2-43780] - Nuf2 antibody [GT312] detects Nuf2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human breast carcinoma.Nuf2 stained by Nuf2 antibody [GT312] (NBP2-43780) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](http://images.novusbio.com/fullsize/nbp2-43780_mouse-monoclonal-nuf2-antibody-312-91020241959357.jpg)
![Western Blot: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP2-32211-img0001.jpg "<b>Biological Strategies Validation. </b>Western Blot: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.")
![<b>Biological Strategies Validation. </b>Immunoprecipitation: Nuf2 Antibody [NBP2-32211] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NUF2 antibody NBP2-32211 used for IP at 6 ug per reaction. For blotting immunoprecipitated NUF2, NBP2-32211 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunoprecipitation-NBP2-32211-img0002.jpg)
![Western Blot: Nuf2 Antibody (644) [NBP2-43779] - Analysis of whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDCA1 antibody [644] at a dilution of 1:1000.](http://images.novusbio.com/fullsize/Nuf2-Antibody-644-Western-Blot-NBP2-43779-img0001.jpg "Western Blot: Nuf2 Antibody (644) [NBP2-43779] - Analysis of whole cell extracts and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with CDCA1 antibody [644] at a dilution of 1:1000.")
![Immunoprecipitation: Nuf2 Antibody (644) [NBP2-43779] - Immunoprecipitation of NUF2 protein from HeLa whole cell extracts using 5 ug of NUF2 antibody [644]. Western blot analysis was performed using NUF2 antibody [644]. EasyBlot anti-Mouse IgG was used as a secondary reagent.](http://images.novusbio.com/fullsize/Nuf2-Antibody-644-Immunoprecipitation-NBP2-43779-img0003.jpg)
![Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).](http://images.novusbio.com/fullsize/Nuf2-Antibody-Western-Blot-NBP2-99081-img0002.jpg "Western Blot: Nuf2 Antibody [NBP2-99081] - Anti-Nuf2 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: Jurkat Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 54 kDa. Observed band size: 54 kDa (We are unsure as to the identity of these extra bands).")
![Immunoprecipitation: Nuf2 Antibody [NBP2-99081] - Nuf2 was immunoprecipitated using: Lane A: 0.5 mg A431 Whole Cell Lysate. Lane B: 0.5 mg Jurkat Whole Cell Lysate. Lane C: 0.5 mg HepG2 Whole Cell Lysate. 1 ul anti-Nuf2 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose. Primary antibody: Anti-Nuf2 rabbit polyclonal antibody, at 1:500 dilution. Secondary antibody: Clean-Bloto IP Detection Reagent (HRP) at 1:500 dilution. Developed using the DAB staining technique. Performed under reducing conditions. Predicted band size: 51 kDa. Observed band size: 51 kDa](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunoprecipitation-NBP2-99081-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP2-55985] - Staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/Nuf2-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-55985-img0001.jpg "Immunocytochemistry/Immunofluorescence: Nuf2 Antibody [NBP2-55985] - Staining of human cell line A-431 shows localization to nucleoplasm.")
![Western Blot: Nuf2 Overexpression Lysate (Adult Normal) [NBL1-13869] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuf2.](http://images.novusbio.com/fullsize/Nuf2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBL1-13869-img0002.jpg "Western Blot: Nuf2 Overexpression Lysate (Adult Normal) [NBL1-13869] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuf2.")
![Western Blot: Nuf2 Overexpression Lysate (Adult Normal) [NBP2-09983] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuf2.](http://images.novusbio.com/fullsize/Nuf2-Overexpression-Lysate-Adult-Normal-Western-Blot-NBP2-09983-img0001.jpg "Western Blot: Nuf2 Overexpression Lysate (Adult Normal) [NBP2-09983] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Nuf2.")
![Western Blot: Nuf2 Antibody (5J0T9) [NBP3-15540] - Western blot analysis of extracts of various cell lines, using Nuf2 Rabbit mAb (NBP3-15540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.](http://images.novusbio.com/fullsize/Nuf2-Antibody-5J0T9-Western-Blot-NBP3-15540-img0001.jpg "Western Blot: Nuf2 Antibody (5J0T9) [NBP3-15540] - Western blot analysis of extracts of various cell lines, using Nuf2 Rabbit mAb (NBP3-15540) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.")
![Western Blot: Nuf2 Antibody (8B7) [NBP3-26452] - Positive Western Blot detected in: Hela whole cell lysate, U251 whole cell lysate. All lanes: Nuf2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55 kDa Observed band size: 55 kDa](http://images.novusbio.com/fullsize/nbp3-26452_rabbit-nuf2-mab-8b7-28220241141368.jpg "Western Blot: Nuf2 Antibody (8B7) [NBP3-26452] - Positive Western Blot detected in: Hela whole cell lysate, U251 whole cell lysate. All lanes: Nuf2 Antibody at 1: 2000 Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 55 kDa Observed band size: 55 kDa")
![Western Blot: Nuf2 Antibody (S07-9E2) [NBP3-19724] - Western blot detection of Nuf2 in K562 cell lysates using NBP3-19724 (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.](http://images.novusbio.com/fullsize/Nuf2-Antibody-S07-9E2-Western-Blot-NBP3-19724-img0001.jpg "Western Blot: Nuf2 Antibody (S07-9E2) [NBP3-19724] - Western blot detection of Nuf2 in K562 cell lysates using NBP3-19724 (1:1000 diluted). Predicted band size: 54kDa. Observed band size: 54kDa.")