Nudel Antibody (9C8T10)

Western Blot: Nudel Antibody (9C8T10) [NBP3-16275] - Western blot analysis of extracts of various cell lines, using Nudel Rabbit mAb (NBP3-16275) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 9C8T10
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

Nudel Antibody (9C8T10) Summary

• Description: Novus Biologicals Rabbit Nudel Antibody (9C8T10) (NBP3-16275) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Nudel (Q9GZM8). MDGEDIPDFSSLKEETAYWKELSLKYKQSFQEARDELVEFQEGSRELEAELEAQLVQAEQRNRDLQADNQRLKYEVEALKEKLEHQYAQSYKQVSVLEDD
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NDEL1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Nudel Antibody (9C8T10)

• DKFZp451M0318
• EOPA
• MITAP1mitosin-associated protein MITAP1
• Mitosin-associated protein 1
• nuclear distribution protein nudE-like 1
• nudE nuclear distribution gene E homolog (A. nidulans)-like 1
• nudE nuclear distribution gene E homolog like 1 (A. nidulans)
• nudE nuclear distribution gene E homolog like 1
• NUDELendooligopeptidase A
• NUDE-like protein
• Protein Nudel

Background

Nudel encodes a thiol-activated peptidase that is phosphorylated in M phase of the cell cycle. Phosphorylation regulates the cell cycle-dependent distribution of this protein, with a fraction of the protein bound strongly to centrosomes in interphase and localized to mitotic spindles in early M phase. Overall, this protein plays a role in nervous system development. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NDEL1