Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free

Western Blot: Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-73086] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Nucleobindin 1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Nucleob

Immunocytochemistry/Immunofluorescence: Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-73086] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Nucleobindin 1.

Immunohistochemistry: Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-73086] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-Nucleobindin 1 mouse monoclonal antibody.

Flow Cytometry: Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-73086] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-Nucleobindin 1 antibody, and then analyzed by flow cytometry.

Western Blot: Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-73086] - Analysis of extracts (10ug) from 9 Human tissue by using anti-NUCB1 monoclonal antibody at 1:200 (1: Testis; 2: Omentum; 3: Uterus; 4: Breast; 5: Brain; 6: Liver; 7: Ovary; 8: Thyroid gland; 9: colon).

Immunohistochemistry: Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free [NBP2-73086] - Staining of paraffin-embedded Human endometrium tissue using anti-Nucleobindin 1 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: OTI1A5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free Summary

• Immunogen: Full length human recombinant protein of human NUCB1(NP_006175) produced in HEk293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NUCB1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
• Theoretical MW: 53.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for Nucleobindin 1 Antibody (OTI1A5) - Azide and BSA Free

• DKFZp686A15286
• FLJ40471
• NUCCALNUC
• nucleobindin 1
• nucleobindin-1

Background

Nucleobindin 1 is a major calcium binding protein of the Golgi. It may have a role in calcium homeostasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NUCB1