NT5C2 Antibody (3C1) - Azide and BSA Free

Western Blot: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of NT5C2 expression in transfected 293T cell line by NT5C2 monoclonal antibody (M02), clone 3C1.Lane 1: NT5C2 transfected lysate(64.97 KDa).Lane 2: Non-transfected lysate.

Immunocytochemistry/Immunofluorescence: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on HeLa cell . Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 3C1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NT5C2 Antibody (3C1) - Azide and BSA Free Summary

• Immunogen: NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE
• Specificity: NT5C2 - 5'-nucleotidase, cytosolic II
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NT5C2
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NT5C2 Antibody (3C1) - Azide and BSA Free

• 5'-nucleotidase (purine), cytosolic type B
• 5'-nucleotidase, cytosolic II
• cN-II
• Cytosolic 5'-nucleotidase II
• EC 3.1.3
• EC 3.1.3.5
• GMP
• NT5BIMP-specific 5'-NT
• NT5CP
• PNT5cytosolic purine 5'-nucleotidase
• purine 5' nucleotidase

Background

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NT5C2 Antibody (H00022978-M02)(8)

Publications using H00022978-M02

• Muhammad-Zawwad R, Octavia C, Charles D et al. CD73 and cN-II regulate the cellular response to chemotherapeutic and hypoxic stress in lung adenocarcinoma cells. Biochim Biophys Acta Gen Subj. 2021-01-09 [PMID: 33434633]
• Cadassou O, Raza MZ, Machon C et al. Enhanced migration of breast and lung cancer cells deficient for cN-II and CD73 via COX-2/PGE2/AKT axis regulation Cell Oncol (Dordr) 2020-09-24 [PMID: 32970317]
• Duarte R, Bachtel N, Cotel M et al. The Psychiatric Risk Gene NT5C2 Regulates Adenosine Monophosphate-Activated Protein Kinase Signaling and Protein Translation in Human Neural Progenitor Cells. Biol Psychiatry. 2019-03-30 [PMID: 31097295]
• Schneider C, Oellerich T, Baldauf HM et al. SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia. Nat Med 2016-12-19 [PMID: 27991919]
• Cividini F, Cros-Perrial E, Pesi R et al. Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II. Int J Biochem Cell Biol. 2015-06-14 [PMID: 26079827]
• Jordheim LP, Puy JY, Cros-Perrial E et al. Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models. Anal Bioanal Chem. 2015-05-22 [PMID: 25998135]
• Cividini F, Tozzi MG, Galli A et al. Cytosolic 5-Nucleotidase II Interacts with the Leucin Rich Repeat of NLR Family Member Ipaf. PLoS One. 2015-03-26 [PMID: 25811392]
• Kulkarni SS, Karlsson HK, Szekeres F et al. Suppression of 5'-Nucleotidase Enzymes Promotes AMP-activated Protein Kinase (AMPK) Phosphorylation and Metabolism in Human and Mouse Skeletal Muscle. J Biol Chem. 2011-08-26 [PMID: 21873433]

Bioinformatics

• Gene Symbol: NT5C2
• Entrez:
  • 22978(Human)
• OMIM:
  • 600417(Human)
• Uniprot:
  • AAH01595(Human)