NT5C2 Antibody (3C1)

Images

 
Western Blot: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of NT5C2 expression in transfected 293T cell line by NT5C2 monoclonal antibody (M02), clone 3C1.Lane 1: NT5C2 transfected lysate(64.97 KDa).Lane 2: ...read more
Immunocytochemistry/ Immunofluorescence: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on HeLa cell . Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NT5C2 Antibody (3C1) [H00022978-M02] - Analysis of monoclonal antibody to NT5C2 on formalin-fixed paraffin-embedded human placenta. Antibody concentration 3 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clone
3C1
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

NT5C2 Antibody (3C1) Summary

Immunogen
NT5C2 (AAH01595, 1 a.a. ~ 561 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSTSWSDRLQNAADMPANMDKHALKKYRREAYHRVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLVSIGYPQELLSFAYDSTFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRDDTERFYILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYKGSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGKVFLATNSDYKYTDKIMTYLFDFPHGPKPGSSHRPWQSYFDLILVDARKPLFFGEGTVLRQVDTKTGKLKIGTYTGPLQHGIVYSGGSSDTICDLLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLVIPELAQELHVWTDKSSLFEELQSLDIFLAELYKHLDSSSNERPDISSIQRRIKKVTHDMDMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLLYYPFSYLFRAAHVLMPHESTVEHTHVDINEMESPLATRNRTSVDFKDTDYKRHQLTRSISEIKPPNLFPLAPQEITHCHDEDDDEEEEEEEE
Specificity
NT5C2 - 5'-nucleotidase, cytosolic II
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NT5C2
Purity
IgG purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
Application Notes
Antibody reactive against transfected lysate and recombinant protein for Western Blot. Has also been used for immunohistochemistry (paraffin) and ELISA.
Publications
Read Publications using H00022978-M02.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NT5C2 Antibody (3C1)

  • 5'-nucleotidase (purine), cytosolic type B
  • 5'-nucleotidase, cytosolic II
  • cN-II
  • Cytosolic 5'-nucleotidase II
  • EC 3.1.3
  • EC 3.1.3.5
  • GMP
  • NT5BIMP-specific 5'-NT
  • NT5CP
  • PNT5cytosolic purine 5'-nucleotidase
  • purine 5' nucleotidase

Background

Purine 5-prime-nucleotidase (EC 3.1.3.5) preferentially hydrolyzes inosine 5-prime-monophosphate (IMP) and other purine nucleotides, and is allosterically activated by various compounds, including ATP. The enzyme is exclusively located in the cytoplasmic matrix of cells and may have a critical role in the maintenance of a constant composition of intracellular purine/pyrimidine nucleotides in cooperation with other nucleotidases.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NT5C2 Antibody (H00022978-M02)(8)


Showing Publications 1 - 8 of 8.
Publications using H00022978-M02 Applications Species
Muhammad-Zawwad R, Octavia C, Charles D et al. CD73 and cN-II regulate the cellular response to chemotherapeutic and hypoxic stress in lung adenocarcinoma cells. Biochim Biophys Acta Gen Subj. 2021-01-09 [PMID: 33434633]
Cadassou O, Raza MZ, Machon C et al. Enhanced migration of breast and lung cancer cells deficient for cN-II and CD73 via COX-2/PGE2/AKT axis regulation Cell Oncol (Dordr) 2020-09-24 [PMID: 32970317]
Duarte R, Bachtel N, Cotel M et al. The Psychiatric Risk Gene NT5C2 Regulates Adenosine Monophosphate-Activated Protein Kinase Signaling and Protein Translation in Human Neural Progenitor Cells. Biol Psychiatry. 2019-03-30 [PMID: 31097295]
Schneider C, Oellerich T, Baldauf HM et al. SAMHD1 is a biomarker for cytarabine response and a therapeutic target in acute myeloid leukemia. Nat Med 2016-12-19 [PMID: 27991919]
Cividini F, Cros-Perrial E, Pesi R et al. Cell proliferation and drug sensitivity of human glioblastoma cells are altered by the stable modulation of cytosolic 5'-nucleotidase II. Int J Biochem Cell Biol. 2015-06-14 [PMID: 26079827]
Jordheim LP, Puy JY, Cros-Perrial E et al. Determination of the enzymatic activity of cytosolic 5'-nucleotidase cN-II in cancer cells: development of a simple analytical method and related cell line models. Anal Bioanal Chem. 2015-05-22 [PMID: 25998135]
Cividini F, Tozzi MG, Galli A et al. Cytosolic 5-Nucleotidase II Interacts with the Leucin Rich Repeat of NLR Family Member Ipaf. PLoS One. 2015-03-26 [PMID: 25811392]
Kulkarni SS, Karlsson HK, Szekeres F et al. Suppression of 5'-Nucleotidase Enzymes Promotes AMP-activated Protein Kinase (AMPK) Phosphorylation and Metabolism in Human and Mouse Skeletal Muscle. J Biol Chem. 2011-08-26 [PMID: 21873433]

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Bioinformatics

Gene Symbol NT5C2
Entrez
OMIM
Uniprot