NT-3 Antibody (OTI5A2)

Western Blot: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Neurotrophin 3 antibody (5A2) at 1:5000 + Mature Recombinant Human Neurotrophin 3 (14KDa).

Immunocytochemistry/Immunofluorescence: Neurotrophin 3 Antibody (5A2) [NBP1-47892] Staining of HeLa cells transiently transfected by pCMV6-ENTRY NTF3.

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded lung using anti-NTF3 mouse monoclonal antibody.

Western Blot: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NTF3(Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-NTF3.

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded Adenocarcinoma of colon using anti-NTF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded breast using anti-NTF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded Carcinoma of kidney using anti-NTF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded Carcinoma of lung using anti-NTF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded Carcinoma of pancreas using anti-NTF3 mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Neurotrophin 3 Antibody (5A2) [NBP1-47892] - Staining of paraffin-embedded kidney using anti-NTF3 mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: OTI5A2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.5 mg/ml

NT-3 Antibody (OTI5A2) Summary

• Immunogen: Recombinant protein expressed in E.coli corresponding to amino acids 139-257 of human NT3
• Specificity: This antibody is specific for Homo sapiens neurotrophin 3 (NTF3), transcript variant 1.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NTF3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin 1:50
  • Western Blot 1:5000
• Theoretical MW: 27.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NT-3 Antibody (OTI5A2)

• HDNF
• MGC129711
• Nerve growth factor 2
• Neurotrophic factor
• neurotrophin 3
• neurotrophin-3
• NGF2
• NGF-2
• NT3
• NT-3
• NTF3

Background

NT3 is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. It is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NT3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NTF3
• Entrez:
  • 4908(Human)