NT-3 Antibody (41512) [Unconjugated]

Recombinant Human NT-3 (Catalog # 267-N3) stimulates proliferation in BaF-TrKB-BD mouse pro-B cell line transfected with TrkB in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human NT-3 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human NT-3 Monoclonal Antibody (Catalog # MAB267). The ND50 is typically 0.1-0.5 µg/mL.

• Applications: B/N, ELISA(Cap)
• Clone: 41512
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

NT-3 Antibody (41512) [Unconjugated] Summary

• Immunogen: S. frugiperda insect ovarian cell line Sf 21-derived recombinant human NT-3
  Tyr139-Thr257
  Accession # P20783
• Specificity: Detects human NT-3 in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) BDNF, rhCNTF, rhGDNF,
  rh beta ‑NGF, and rhNT-4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NTF3
• Purity Statement: Protein A or G purified from ascites
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Neutralization 0.1-0.5 ug/mL
• Application Notes: ELISA Detection: Human NT-3 Biotinylated Antibody (Catalog number BAF267)
  Standard: Recombinant Human NT-3 (Catalog number 267-N3)

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NT-3 Antibody (41512) [Unconjugated]

• HDNF
• MGC129711
• Nerve growth factor 2
• Neurotrophic factor
• neurotrophin 3
• neurotrophin-3
• NGF2
• NGF-2
• NT3
• NT-3
• NTF3

Background

Neurotrophin-3 (NT-3) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous systems. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins. All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive NT-3 is predicted to be a non-covalently linked homodimer.

The NT-3 cDNA encodes a 257 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature NT-3. The amino acid sequence of mature NT-3 is identical in human, mouse and rat. NT-3 transcripts have been detected in the cerebellum, hippocampus, placenta, heart, skin, and skeletal muscle. NT-3 primarily activates the TrkC tyrosine kinase receptor. In addition, NT-3 can activate Trk and TrkB kinase receptors in certain cell systems. NT-3 can also bind with low affinity to the low affinity NGF receptor.

1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
2. Snider, W.D. (1994) Cell 77:627.
3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NT-3 Antibody (MAB267)(4)

Publications using MAB267

• Sahenk Z, Galloway G, Clark K, Malik V, Rodino-Klapac L, Kaspar B, Chen L, Braganza C, Montgomery C, Mendell J AAV1.NT-3 gene therapy for charcot-marie-tooth neuropathy. Mol Ther, 2013-10-28;22(3):511-21. 2013-10-28 [PMID: 24162799] (ELISA Development (Capture), Human)
• Bouzas-Rodriguez J, Cabrera JR, Delloye-Bourgeois C, Ichim G, Delcros JG, Raquin MA, Rousseau R, Combaret V, Benard J, Tauszig-Delamasure S, Mehlen P Neurotrophin-3 production promotes human neuroblastoma cell survival by inhibiting TrkC-induced apoptosis. J. Clin. Invest., 2010-02-15;120(3):850-8. 2010-02-15 [PMID: 20160348] (ELISA Development, Human)
• Samah B, Porcheray F, Gras G Neurotrophins modulate monocyte chemotaxis without affecting macrophage function. Clin. Exp. Immunol., 2008-01-08;151(3):476-86. 2008-01-08 [PMID: 18190610] (ICC, Human)
• Nassenstein C, Braun A, Erpenbeck VJ, Lommatzsch M, Schmidt S, Krug N, Luttmann W, Renz H, Virchow JC The neurotrophins nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3, and neurotrophin-4 are survival and activation factors for eosinophils in patients with allergic bronchial asthma. J. Exp. Med., 2003-08-04;198(3):455-67. 2003-08-04 [PMID: 12900521] (Neutralization, Human)

Bioinformatics

• Gene Symbol: NTF3
• Uniprot:
  • P20783()