NSUN2 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: NSUN2 Antibody - BSA Free [NBP3-47302] - Immunofluorescent analysis of Hela cells using (NSUN2 antibody) at dilution of 1:25

Immunohistochemistry: NSUN2 Antibody - BSA Free [NBP3-47302] - Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using (NSUN2 Antibody) at dilution of 1:200 heat mediated antigen retrieved with Tris-EDTA buffer(NSUN2

Western Blot: NSUN2 Antibody - BSA Free [NBP3-47302] - HepG2 cells cells were subjected to SDS PAGE followed by western blot with (NSUN2 antibody) at dilution of 1:5000

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NSUN2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NSUN2 Antibody - BSA Free (NBP3-47302) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: NOL1/NOP2/Sun domain family, member 2. (Uniprot# Q08J23)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NSUN2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:20-1:200
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:500-1:2000
• Application Notes: Theoretical Molecular Weight is 90-100 kDa

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for NSUN2 Antibody - BSA Free

• EC 2.1.1
• EC 2.1.1.29
• FLJ20303,5-methycytoisine methyltransferase
• hTrm4
• member 2
• Myc-induced SUN-domain-containing protein
• NOL1/NOP2/Sun domain family 2
• NOL1/NOP2/Sun domain family member 2
• NOP2/Sun domain family, member 2
• SAKIMisu
• Substrate of AIM1/Aurora kinase B
• TRM4MISU
• tRNA (cytosine-5-)-methyltransferase NSUN2
• tRNA (cytosine-5-)-methyltransferase
• tRNA methyltransferase 4 homolog

Background

Maturation of cytoplasmic tRNAs includes splicing of introns, which are located 1 nucleotide 3-prime from the anticodon in all intron-containing tRNA genes. In tRNA-leu(CAA), the first position of the anticodon, C34, is converted to 5-methylcytosine, a modification necessary to stabilize the anticodon-codon pairing and correctly translate the mRNA. NSUN2 encodes a methyltransferase that catalyzes the intron-dependent formation of 5-methylcytosine at C34 of tRNA-leu(CAA) (Brzezicha et al., 2006 [PubMed 17071714]).[supplied by OMIM].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NSUN2