Western Blot: NRSF Antibody [NB100-757] - Detection of Human REST on HeLa whole cell lysate using NB100-757. REST was most efficiently immunoprecipitated using rabbit anti-REST antibody NB100-756 at 3 ug/mg lysate.
Immunohistochemistry-Paraffin: NRSF Antibody [NB100-757] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: NRSF Antibody [NB100-757] - Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti-REST (Cat. No. NB100-757, Lot1) used at a dilution of 1:1,000 ...read more
The immunogen recognized by this antibody maps to a region between residue 1050 and the C-terminus (residue 1097) of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).
Monkey (100%), Primate (100%)
Immunogen affinity purified
Test in a species/application not listed above to receive a full credit towards a future purchase.
This antibody can be used in Western Blot and Immunoprecipitation, IHC-P and IF. Recommended Starting Dilutions*: WB: 1:2,500-1:10,000 IP: 2-5 ug/mg Immunoprecipitation 2 to 5 ug/mg lysate. NB100-757 is less efficient than NB100-756 at IP Immunohistochemistry 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. *Optimal dilutions should be determined by the end user.
Read 1 Review rated 3 using NB100-757 in the following application:
Based on 100% sequence identity, this antibody is predicted to react with Orangutan, Rhesus Monkey, Gorilla, Chimpanzee, White-tufted-ear marmoset, Crab-eating macaque and Northern white-cheeked gibbon.
Alternate Names for NRSF Antibody
Neural-restrictive silencer factor
NRSFneuron restrictive silencer factor
RE1-silencing transcription factor
X2 box repressor
XBRrepressor binding to the X2 box
REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.