Biological Strategies: Western Blot: NRSF Antibody [NB100-757] - Detection of Human REST by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. ...read more
Immunohistochemistry-Paraffin: NRSF Antibody [NB100-757] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: NRSF Antibody [NB100-757] - Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti-REST (Cat. No. NB100-757, Lot1) used at a dilution of 1:1,000 ...read more
The immunogen recognized by this antibody maps to a region between residue 1050 and the C-terminus (residue 1097) of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).
Immunogen affinity purified
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REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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