NRSF Antibody

Images

 
Western Blot: NRSF Antibody [NB100-757] - Detection of Human REST by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and Jurkat cells prepared using NETN lysis buffer. Antibodies: Affinity purified ...read more
Immunohistochemistry-Paraffin: NRSF Antibody [NB100-757] - FFPE section of human ovarian carcinoma. Antibody used at a dilution of 1:250. Detection: DAB staining using Immunohistochemistry Accessory Kit.
Immunohistochemistry-Paraffin: NRSF Antibody [NB100-757] - Sample: FFPE section of human metastatic lymph node. Antibody: Affinity purified rabbit anti-REST (Cat. No. NB100-757, Lot1) used at a dilution of 1:1,000 ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
   

Biological Strategies

     

Order Details

NRSF Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1050 and the C-terminus (residue 1097) of human RE1-silencing transcription factor using the numbering given in entry NP_005603.2 (GeneID 5978).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
REST
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:10000
  • Immunohistochemistry-Paraffin 1:500-1:2000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
Positive Control
NRSF Lysate (NBL1-15286)
Reviewed Applications
Read 1 Review rated 3
using
NB100-757 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for NRSF Antibody

  • Neural-restrictive silencer factor
  • NRSFneuron restrictive silencer factor
  • RE1-silencing transcription factor
  • X2 box repressor
  • XBRrepressor binding to the X2 box

Background

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu
Applications: WB, IHC-P

Publications for NRSF Antibody (NB100-757) (0)

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Review for NRSF Antibody (NB100-757) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NB100-757:
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WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot NRSF NB100-757
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reviewed by:
Anonymous
WB Human 04/02/2015
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Summary

ApplicationWestern Blot
Sample TestedLNCaP prostate cancer cells
SpeciesHuman
LotA2

Blocking

Blocking Details5% Milk in PBST, room temperature for 1 hr

Primary Anitbody

Dilution Ratio1:5000 in PBST containing 5% milk. Incubated at 4C overnight

Secondary Antibody

Secondary DescriptionGoat Anti-mouse IgG-HRP
Secondary Manufacturer Cat#sc-2005
Secondary Concentration1:10,000 diluted in PBST containing 5% milk, incubated for 1 hr at room temp

Details

Detection NotesChemiluminescence using luminol and peroxidase solution. Visualized with Fujifilm LAS4000, exposure time-5 seconds

Comments

CommentsThere are a couple of nonspecific band of lower size

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FAQs for NRSF Antibody (NB100-757) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for NRSF Antibody (NB100-757)

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Recent Reviews

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1
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Anonymous
04/02/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol REST
Entrez
Uniprot