NRSF Antibody (SR1230) - BSA Free

Western Blot: NRSF Antibody (SR1230) [NBP3-21822] - Western blot analysis using NBP3-21822 in HeLa cell lysate.

• Reactivity: Hu
• Applications: WB
• Clone: SR1230
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NRSF Antibody (SR1230) - BSA Free Summary

• Description: Novus Biologicals Rabbit NRSF Antibody (SR1230) - BSA Free (NBP3-21822) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human NRSF (Uniprot #: Q13127)
• Specificity: Detects endogenous levels of total NRSF
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: REST
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:2000
• Theoretical MW: 160 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NRSF Antibody (SR1230) - BSA Free

• Neural-restrictive silencer factor
• NRSFneuron restrictive silencer factor
• RE1-silencing transcription factor
• X2 box repressor
• XBRrepressor binding to the X2 box

Background

REST, which is short for RE1-silencing transcription factor, locks down neuronal genes in other cells by grabbing onto the DNA and cementing in other molecules, an arrangement that stays intact as non-neuronal cells differentiate into liver, muscle, and other tissues. REST uses a different temporary off mechanism to direct neuronal development. REST is a tumor suppressor gene in epithelial cells and has been found to be mutated in 33 percent of colorectal tumors and cell lines studied. Rest could eventually serve as a marker for diagnosing colorectal and other types of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: REST