NR4A3/NOR1 Antibody (1E9) - Azide and BSA Free

Western Blot: NR4A3/NOR1 Antibody (1E9) [H00008013-M02] - NR4A3 monoclonal antibody (M02), clone 1E9 Analysis of NR4A3 expression in Hela S3 NE.

Immunocytochemistry/Immunofluorescence: NR4A3/NOR1 Antibody (1E9) [H00008013-M02] - Analysis of monoclonal antibody to NR4A3 on HeLa cell. Antibody concentration 10 ug/ml.

Sandwich ELISA: NR4A3/NOR1 Antibody (1E9) [H00008013-M02] - Detection limit for recombinant GST tagged NR4A3 is approximately 0.3ng/ml as a capture antibody.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1E9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NR4A3/NOR1 Antibody (1E9) - Azide and BSA Free Summary

• Description: Quality control test: Antibody Reactive Against Recombinant Protein.
• Immunogen: NR4A3 (NP_008912, 414 a.a. ~ 521 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LDYSRYCPTDQAAAGTDAEHVQQFYNLLTASIDVSRSWAEKIPGFTDLPKEDQTLLIESAFLELFVLRLSIRSNTAEDKFVFCNGLVLHRLQCLRGFGEWLDSIKDFS
• Specificity: NR4A3 - nuclear receptor subfamily 4, group A, member 3 (1E9)
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR4A3
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Sandwich ELISA
  • Western Blot
• Application Notes: Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IF and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for NR4A3/NOR1 Antibody (1E9) - Azide and BSA Free

• CHN
• CSMF
• MINOR
• NOR1
• TEC

Background

This gene encodes a member of the steroid-thyroid hormone-retinoid receptor superfamily. The encoded protein may act as a transcriptional activator. The protein can efficiently bind the NGFI-B Response Element (NBRE). Three different versions of extraskeletal myxoid chondrosarcomas (EMCs) are the result of reciprocal translocations between this gene and other genes. The translocation breakpoints are associated with Nuclear Receptor Subfamily 4, Group A, Member 3 (on chromosome 9) and either Ewing Sarcome Breakpoint Region 1 (on chromosome 22), RNA Polymerase II, TATA Box-Binding Protein-Associated Factor, 68-KD (on chromosome 17), or Transcription factor 12 (on chromosome 15). Four transcript variants encoding three distinct isoforms have been identified for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NR4A3/NOR1 Antibody (H00008013-M02)(1)

Publication using H00008013-M02

• Dadheech N, Villela BS, Rouillard C, and Buteau J The Orphan Nuclear Receptor Nor1/Nr4a3 Is a Negative Regulator of beta -Cell Mass. J Biol Chem. 2019-01-29 [PMID: 30696767]

Bioinformatics

• Gene Symbol: NR4A3
• Entrez:
  • 8013(Human)
• Uniprot:
  • NP_008912(Human)