NPM1 [p Thr199] Antibody (SR1550) - BSA Free

Western Blot: NPM1 [p Thr 199] Antibody (SR1550) [NBP3-21600] - Western blot analysis using NBP3-21600 in (1) HeLa cell lysate; (2) HeLa cell treated with CA lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SR1550
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NPM1 [p Thr199] Antibody (SR1550) - BSA Free Summary

• Description: Novus Biologicals Rabbit NPM1 [p Thr199] Antibody (SR1550) - BSA Free (NBP3-21600) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human phospho-NPM1 [Thr199] (Uniprot #: P06748)
• Modification: p Thr199
• Specificity: Detects endogenous levels of total phospho-NPM1 [Thr199]
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NPM1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NPM1 [p Thr199] Antibody (SR1550) - BSA Free

• B23
• MGC104254
• NPM1
• NPMnucleophosmin/nucleoplasmin family, member 1
• Nucleolar phosphoprotein B23
• Nucleolar protein NO38
• nucleophosmin (nucleolar phosphoprotein B23, numatrin)
• Nucleophosmin
• Numatrin

Background

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NPM1