Npas4 Antibody - Azide and BSA Free

• Reactivity: Mu, Rt, Sh
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

Npas4 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Npas4 Antibody - Azide and BSA Free (NBP2-47252) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Npas4 Antibody: Cited in 5 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide derived from Cter domain of mouse Npas4 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPAS4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:500
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Frozen 1:100 - 1:1000
  • Immunohistochemistry-Paraffin
  • Western Blot 1:1000 - 1:5000
• Application Notes: Use in IHC-P reported in scientific literature (PMID: 23598442).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 0.1M Tris , 0.1M Glycine and 2% Sucrose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Npas4 Antibody - Azide and BSA Free

• BHLHE79
• bHLHe79neuronal PAS domain-containing protein 4
• Class E basic helix-loop-helix protein 79
• Le-PAS
• neuronal PAS domain protein 4
• Neuronal PAS4
• NXFHLH-PAS transcription factor NXF
• PASD10PAS domain-containing protein 10

Background

Npas4 acts as a transcriptional activator in the presence of ARNT. It can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Npas4 Antibody (NBP2-47252)(5)

Publications using NBP2-47252

• Ma L, Wang F, Li Y et al. Brain methylome remodeling selectively regulates neuronal activity genes linking to emotional behaviors in mice exposed to maternal immune activation Nature communications 2023-11-29 [PMID: 38030616] (ICC/IF, Mouse)
• Wang XM, Zhang GF, Jia M et al. Environmental enrichment improves pain sensitivity, depression-like phenotype, and memory deficit in mice with neuropathic pain: role of NPAS4 Psychopharmacology (Berl.) 2019-02-23 [PMID: 30798405] (WB, Mouse)
• West A, Dupre S, Yu L, Paton I et al. Npas4 is activated by melatonin, and drives the clock gene Cry1 in the ovine pars tuberalis. Mol Endocrinol. [PMID: 23598442] (IHC-P, IF/IHC, ICC/IF, Sheep)
  
  Details:
  Citation using the Unpurified form of this antibody.
• Wakasaya Y, Kawarabayashi T, Watanabe M et al. Factors responsible for neurofibrillary tangles and neuronal cell losses in tauopathy. J Neurosci Res. [PMID: 21312224]
  
  Details:
  Citation using the Unpurified form of this antibody.

Bioinformatics

• Gene Symbol: NPAS4
• Entrez:
  • 225872(Mouse)
• Uniprot:
  • Q8BGD7(Mouse)