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![Western Blot: Npas4 Antibody (S408-79) [NBP2-59332] - Western Blot analysis of Rat Brain showing detection of ~90 kDa Npas4 protein using Mouse Anti-Npas4 Monoclonal Antibody, Clone S408-79 (NBP2-59332). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Npas4 Monoclonal Antibody (NBP2-59332) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.](http://images.novusbio.com/fullsize/Npas4-Antibody-S408-79-Western-Blot-NBP2-59332-img0005.jpg "Western Blot: Npas4 Antibody (S408-79) [NBP2-59332] - Western Blot analysis of Rat Brain showing detection of ~90 kDa Npas4 protein using Mouse Anti-Npas4 Monoclonal Antibody, Clone S408-79 (NBP2-59332). Lane 1: MW Ladder. Lane 2: Rat Brain. Load: 20 ug. Block: 2% GE Healthcare Blocker for 1 hour at RT. Primary Antibody: Mouse Anti-Npas4 Monoclonal Antibody (NBP2-59332) at 1:1000 for 16 hours at 4C. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~90 kDa. Other Band(s): ~60, 45, 40, 38, 25, 20 kDa.")
![Immunocytochemistry/Immunofluorescence: Npas4 Antibody (S408-79) [NBP2-59332] - Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-NPAS4 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) NPAS4 Antibody (D) Composite.](http://images.novusbio.com/fullsize/Npas4-Antibody-S408-79-Immunocytochemistry-Immunofluorescence-NBP2-59332-img0004.jpg)
![Western Blot: Npas4 Antibody [NBP1-68863] - Staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Npas4-Antibody-Western-Blot-NBP1-68863-img0002.jpg "Western Blot: Npas4 Antibody [NBP1-68863] - Staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.")
![Western Blot: Npas4 Antibody [NBP1-68863] - NBP1-68863 (0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.](http://images.novusbio.com/fullsize/Npas4-Antibody-Western-Blot-NBP1-68863-img0001.jpg)
![SDS-Page: Recombinant Human Npas4 Protein [H00266743-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.](http://images.novusbio.com/fullsize/Recombinant-Human-Npas4-Protein-SDS-Page-H00266743-Q01-img0001.jpg "SDS-Page: Recombinant Human Npas4 Protein [H00266743-Q01] - 12.5% SDS-PAGE Stained with Coomassie Blue.")
![Western Blot: Npas4 Antibody [NBP3-12478] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 96, 60 and 38 KDa.](http://images.novusbio.com/fullsize/Npas4-Antibody-Western-Blot-NBP3-12478-img0001.jpg "Western Blot: Npas4 Antibody [NBP3-12478] - 1:500 antibody dilution in DiluObuffer. Apparent MW is 96, 60 and 38 KDa.")
![ELISA: Npas4 Antibody (4E4) [H00266743-M09] - Detection limit for recombinant GST tagged NPAS4 is 0.3 ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Npas4-Antibody-4E4-ELISA-H00266743-M09-img0002.jpg "ELISA: Npas4 Antibody (4E4) [H00266743-M09] - Detection limit for recombinant GST tagged NPAS4 is 0.3 ng/ml as a capture antibody.")