Npas4 Antibody

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Product Details

Summary
Reactivity Mu, Rt, ShSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

Npas4 Antibody Summary

Immunogen
Synthetic peptide derived from Cterm domain of mouse NPAS4 protein
Specificity
Reacts specifically with mouse 87 kDa protein
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NPAS4
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000-1:5000
  • ELISA 1:100-1:2000
  • Immunocytochemistry/Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Control Peptide
Npas4 Protein (NBP1-06574PEP)
Reviewed Applications
Read 1 Review rated 3
using
NBP1-06574 in the following applications:

Publications
Read Publications using
NBP1-06574 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Antiserum
Preservative
No Preservative
Concentration
LYOPH
Purity
Unpurified
Reconstitution Instructions
Reconstitute with 0.2 ml distilled water.

Alternate Names for Npas4 Antibody

  • BHLHE79
  • bHLHe79neuronal PAS domain-containing protein 4
  • Class E basic helix-loop-helix protein 79
  • Le-PAS
  • neuronal PAS domain protein 4
  • Neuronal PAS4
  • NXFHLH-PAS transcription factor NXF
  • PASD10PAS domain-containing protein 10

Background

Npas4 acts as a transcriptional activator in the presence of ARNT. It can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Npas4 Antibody (NBP1-06574)(2)

We have publications tested in 1 confirmed species: Sheep.

We have publications tested in 3 applications: ICC/IF, IHC, IHC-P.


Filter By Application
ICC/IF
(1)
IHC
(1)
IHC-P
(1)
All Applications
Filter By Species
Sheep
(1)
All Species

Review for Npas4 Antibody (NBP1-06574) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Rat.

Reviews using NBP1-06574:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Rat
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Npas4 NBP1-06574
Enlarge
reviewed by:
Anonymous
WB Rat 07/02/2013
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Summary

ApplicationWestern Blot
Sample TestedRat brain lysate (ventral hippocampus)
SpeciesRat
Lot12H3
CommentsDespite some background, the immunostaining observed was sufficient for a reliable quantification.

Blocking

Blocking DetailsBlocking in TBS with 3% milk for 1h at RT

Primary Anitbody

Dilution Ratio1:2000 in TBST with 3% milk

Secondary Antibody

Secondary DescriptionGoat anti-rabbit
Secondary Manufacturer Cat#Santa Cruz
Secondary Concentration1:4,000

Details

Detection NotesECL Pico kit (Amersham) for 2min

Comments

CommentsDespite some background, the immunostaining observed was sufficient for a reliable quantification.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Npas4 Antibody (NBP1-06574). (Showing 1 - 2 of 2 FAQs).

  1. May we ask if any IHC image produced by NBP1-06574 can be provided for a reference?
    • Unfortunately we do not have an IHC image for the Npas4 antibody, catalog number NBP1-06574, however we do stand behind the use of this antibody in IHC with a 100% guarantee.
  2. I would like to know the sequence of the synthetic peptide used to make the Npas4 antibody (cat. # NBP1-06574). It says on the product sheet that it was raised against the C-terminus, but I would like to know the specific sequence, if it is known and available.
    • The exact sequence of NBP1-06574 is proprietary, but I can offer you the amino acid range. The immunogen falls between amino acids 600- 700 of Mouse Npas4 (accession number Q8BGD7).

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NBP1-06574

Bioinformatics Tool for Npas4 Antibody (NBP1-06574)

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Diseases for Npas4 Antibody (NBP1-06574)

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Pathways for Npas4 Antibody (NBP1-06574)

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Blogs on Npas4

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Contact Information

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Calculators

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Recent Reviews

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Anonymous
07/02/2013
Application: WB
Species: Rat

Bioinformatics

Gene Symbol NPAS4
Entrez
Uniprot