Npas4 Antibody - Unpurified

• Reactivity: Mu, Rt, Sh
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Unpurified
• Concentration: LYOPH

Npas4 Antibody - Unpurified Summary

• Description: Novus Biologicals Rabbit Npas4 Antibody - Unpurified (NBP2-47252) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-Npas4 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide derived from Cterm domain of mouse NPAS4 protein
• Specificity: Reacts specifically with mouse 87 kDa protein
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NPAS4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 1:10-1:500
  • Immunohistochemistry 1:100 - 1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:5000
• Control Peptide:

  Npas4 Protein (NBP1-06574PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Antiserum
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Unpurified
• Reconstitution Instructions: Lyophilized powder, reconstituted in distilled water.

Alternate Names for Npas4 Antibody - Unpurified

• BHLHE79
• bHLHe79neuronal PAS domain-containing protein 4
• Class E basic helix-loop-helix protein 79
• Le-PAS
• neuronal PAS domain protein 4
• Neuronal PAS4
• NXFHLH-PAS transcription factor NXF
• PASD10PAS domain-containing protein 10

Background

Npas4 acts as a transcriptional activator in the presence of ARNT. It can activate the CME (CNS midline enhancer) element and the expression of the drebrin gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Npas4 Antibody (NBP1-06574)(2)

Publications using NBP1-06574

• West A, Dupre S, Yu L, Paton I et al. Npas4 is activated by melatonin, and drives the clock gene Cry1 in the ovine pars tuberalis. Mol Endocrinol. [PMID: 23598442] (IHC-P, ICC/IF, IF/IHC, Sheep)
  
  Details:
  Citation using the Unpurified form of this antibody.
• Wakasaya Y, Kawarabayashi T, Watanabe M et al. Factors responsible for neurofibrillary tangles and neuronal cell losses in tauopathy. J Neurosci Res. [PMID: 21312224]
  
  Details:
  Citation using the Unpurified form of this antibody.

Review for Npas4 Antibody (NBP1-06574) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Verified Customer WB Rat 07/02/2013

Summary

• Application: Western Blot
• Sample Tested: Rat brain lysate (ventral hippocampus)
• Species: Rat
• Lot: 12H3

FAQs for Npas4 Antibody (NBP1-06574). (Showing 1 - 2 of 2 FAQs).

1. May we ask if any IHC image produced by NBP1-06574 can be provided for a reference?
   • Unfortunately we do not have an IHC image for the Npas4 antibody, catalog number NBP1-06574, however we do stand behind the use of this antibody in IHC with a 100% guarantee.
2. I would like to know the sequence of the synthetic peptide used to make the Npas4 antibody (cat. # NBP1-06574). It says on the product sheet that it was raised against the C-terminus, but I would like to know the specific sequence, if it is known and available.
   • The exact sequence of NBP1-06574 is proprietary, but I can offer you the amino acid range. The immunogen falls between amino acids 600- 700 of Mouse Npas4 (accession number Q8BGD7).

Bioinformatics

• Gene Symbol: NPAS4
• Entrez:
  • 225872(Mouse)
• Uniprot:
  • Q8BGD7(Mouse)