Nox4 Antibody [FITC]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, PmSpecies Glossary
Applications Flow, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
FITC

Nox4 Antibody [FITC] Summary

Immunogen
A synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein sequence. [Swiss-Prot# Q9NPH5].
Localization
Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NOX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
Application Notes
This NOX4 antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and Immunohistochemistry paraffin embedded sections. In Western Blot this NOX4 antibody recognizes bands at ~67kDa and ~31 kDa, representing isoforms 1 and 4, respectively.

Reactivity Notes

Human, mouse, rat, pig and primate.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Nox4 Antibody [FITC]

  • EC 1.6.3
  • Kidney oxidase-1
  • Kidney superoxide-producing NADPH oxidase
  • KOX
  • KOX-1
  • KOX-1Renal NAD(P)H-oxidase
  • NADPH oxidase 4
  • Nox4
  • RENOX
  • RENOXEC 1.6.3.-

Background

NOX4 (NADPH oxidase 4) is a constitutive NADPH oxidase which complex with CYBA/p22phox for generating superoxide intracellularly and is found primarily in vascular cells, fibroblasts, osteoclasts and is abundantly expressed in the kidney. NOX4 regulates signaling cascades through phosphatases inhibition and function as oxygen sensor regulating the KCNK3/TASK-1 potassium channel as well as HIF1 alpha activity. NOX4 has also been implicated in regulation of insulin signaling cascade, apoptosis, bone resorption and LPS-mediated activation of NFkB. In vascular smooth muscle cells, NOX4 is positively regulated by Poldip2 which is a Nox4/p22phox interacting protein and Nox4/p22phox-Poldip2 complex regulates Rho-dependent cytoskeletal reorganization as well as cellular migration. TGF- beta is another positive regulator of Nox4 which is important in vascular fibrosis and differentiation. Unlike Nox1/Nox2, Nox4 is constitutively active, producing primarily H2O2 rather thanO2 and this difference in the species generated underlie NOX-specific actions in cell signaling. Nox4 contributes to basal ROS production through its constitutive activity and to increased ROS generation when stimulated by Ang II, glucose, TNF alpha and growth factors. NOX4 has been implicated in hypertension, atherosclerosis, and cardiovascular and renal complications of diabetes and in remodeling of pulmonary arteries in hypertension.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

FAQs for Nox4 Antibody (NB110-58851F) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for Nox4 Antibody (NB110-58851F)

Discover related pathways, diseases and genes to Nox4 Antibody (NB110-58851F). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for Nox4 Antibody (NB110-58851F)

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Blogs on Nox4.

NOX4 Antibodies: Don't NOX them until you've tried them
NOX4 is an NADPH oxidase that generates superoxide within the cell. It is primarily found in vascular cells, fibroblasts, and osteoclasts, with abundant expression in the kidney. Unlike its family members NOX1 and NOX2, NOX4 is constitutively active,...  Read full blog post.

NOX4 Antibodies in Diabetic Nephropathy Research
Diabetic nephropathy (DN) is one of the leading complications resulting from chronic diabetes. It manifests as progressive renal failure caused by mesangial cell hyperplasia and fibrosis, and is one of the leading causes of terminal kidney disease (1)...  Read full blog post.

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Bioinformatics

Gene Symbol NOX4
Entrez