Nox4 Antibody (996715) [Unconjugated]

Nox4 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs; left panel) using Mouse Anti-Human Nox4 Monoclonal Antibody (Catalog # MAB8158) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. MCF-7 human breast cancer cell line is shown as negative staining (right panel). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Nox4 was detected in immersion fixed paraffin-embedded sections of human kidney using Mouse Anti-Human Nox4 Monoclonal Antibody (Catalog # MAB8158) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Hu
• Applications: IHC, ICC/IF
• Clone: 996715
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Nox4 Antibody (996715) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human NOX4
  Lys450-Ser578
  Accession # Q9NPH5
• Specificity: Detects human NOX4 in direct ELISAs.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 5-25 ug/mL
  • Immunohistochemistry 1-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Nox4 Antibody (996715) [Unconjugated]

• EC 1.6.3
• Kidney oxidase-1
• Kidney superoxide-producing NADPH oxidase
• KOX
• KOX-1
• KOX-1Renal NAD(P)H-oxidase
• NADPH oxidase 4
• Nox4
• RENOX
• RENOXEC 1.6.3.-

Background

NADPH Oxidase 4/NOX4, also known as Renal NADPH Oxidase/RENOX and Kidney Oxidase 1/KOX1, is a 66 to 75 kDa member of the NOX family of NADPH oxidases. NOX family members are a major source of reactive oxygen species (ROS), including hydrogen peroxide and superoxide, and are critical mediators of redox signaling. NOX4 is strongly expressed in the kidney, especially in the proximal convoluted tubule cells of the renal cortex, and may function as an oxygen sensor that regulates the synthesis of erythropoietin. Expression is also high in endothelial cells, and because aortas from deficient mice exhibit increased inflammation, hypertrophy and endothelial dysfunction, NOX4 may function to protect the vasculature during ischemic or inflammatory stress. Human NOX4 is 578 amino acids (aa) in length, with several additional smaller isoforms identified. Over aa 450-578, human NOX4 has 92% sequence identity with mouse NOX4, and 91% identity with rat NOX4.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Nox4 Antibody (MAB8158)(1)

Publication using MAB8158

• Kuehl, M;Okabayashi, Y;Wong, MN;Gernhold, L;Gut, G;Kaiser, N;Schwerk, M;Gräfe, SK;Ma, FY;Tanevski, J;Schäfer, PSL;Mezher, S;Sarabia Del Castillo, J;Goldbeck-Strieder, T;Zolotareva, O;Hartung, M;Delgado Chaves, FM;Klinkert, L;Gnirck, AC;Spehr, M;Fleck, D;Joodaki, M;Parra, V;Shaigan, M;Diebold, M;Prinz, M;Kranz, J;Kux, JM;Braun, F;Kretz, O;Wu, H;Grahammer, F;Heins, S;Zimmermann, M;Haas, F;Kylies, D;Wanner, N;Czogalla, J;Dumoulin, B;Zolotarev, N;Lindenmeyer, M;Karlson, P;Nyengaard, JR;Sebode, M;Weidemann, S;Wiech, T;Groene, HJ;Tomas, NM;Meyer-Schwesinger, C;Kuppe, C;Kramann, R;Karras, A;Bruneval, P;Tharaux, PL;Pastene, D;Yard, B;Schaub, JA;McCown, PJ;Pyle, L;Choi, YJ;Yokoo, T;Baumbach, J;Sáez, PJ;Costa, I;Turner, JE;Hodgin, JB;Saez-Rodriguez, J;Huber, TB;Bjornstad, P;Kretzler, M;Lenoir, O;Nikolic-Paterson, DJ;Pelkmans, L;Bonn, S;Puelles, VG; Pathology-oriented multiplexing enables integrative disease mapping Nature 2025-07-18 [PMID: 40681898] (IF/IHC, Human)

Bioinformatics

• Uniprot:
  • Q9NPH5()