NOX1 Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: NOX1 Antibody [NBP2-93835] - Human colon carcinoma using NOX1 antibody at dilution of 1:100 (40x lens).

Western Blot: NOX1 Antibody - Azide and BSA Free [NOX1] - Western blot analysis of lysates from Mouse liver, using NOX1 Rabbit pAb at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 30s.

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

NOX1 Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit NOX1 Antibody - Azide and BSA Free (NBP2-93835) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 418-564 of human NOX1 (NP_008983.2). WYKFQCADHNLKTKKIYFYWICRETGAFSWFNNLLTSLEQEMEELGKVGFLNYRLFLTGWDSNIVGHAALNFDKATDIVTGLKQKTSFGRPMWDNEFSTIATSHPKSVVGVFLCGPRTLAKSLRKCCHRYSSLDPRKVQFYFNKENF
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NOX1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:200 - 1:500

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.05% Proclin 300
• Purity: Affinity purified

Alternate Names for NOX1 Antibody - Azide and BSA Free

• EC 1.6.3
• GP91-2
• Mitogenic oxidase 1
• MOX-1
• MOX1NADH/NADPH mitogenic oxidase subunit P65-MOX
• NADPH oxidase 1
• NADPH oxidase homolog-1
• NOH1
• NOH1mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)
• NOH-1NADPH oxidase 1 variant NOH-1L
• NOX1
• NOX-1

Background

The NOX family NADPH protein oxidases are involved in transfer of electrons accross membranes to generate reactive oxygen species. There are 7 family members - NOX1-5, DUOX1 and DUOX2. Expression of NOX1 is highest in colon tissue; it is also found in vascular tissue and can also been induced by a variety of factors including PDGF. It requires interaction with NOXO1 and NOXA1 for superoxide generation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: NOX1