Notch1b Antibody (SJ205)

Western Blot: Notch1b Antibody (SJ205) [NBP2-67420] - Western blot analysis of Notch1b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-R

Immunocytochemistry/Immunofluorescence: Notch1b Antibody (SJ205) [NBP2-67420] - Staining Notch 1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Notch1b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-55, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Flow Cytometry: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of Hela cells with Notch 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Western Blot: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of Notch 1 on HepG2 cell lysates using anti-Notch 1 antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Notch1b Antibody (SJ205) [NBP2-67420] - Staining Notch 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Notch1b Antibody (SJ205) [NBP2-67420] - Staining Notch 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded human lung tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded human spleen tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded mouse lung tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded mouse spleen tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Notch1b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ET1606-55, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

Notch1b Antibody (SJ205) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Notch1b aa 2,481-2,530 / 2,555. (SwissProt: P46531 Human; SwissProt: Q01705 Mouse)
• Localization: Cell membrane, Nucleus(intracellular domain).
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: NOTCH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Notch1b Antibody (SJ205)

• EC 2.1.2.11
• EC 3.4.21.68
• hN1
• Notch (Drosophila) homolog 1 (translocation-associated)
• notch 1Notch homolog 1, translocation-associated (Drosophila)
• Notch homolog 1, translocation-associated
• TAN1neurogenic locus notch homolog protein 1
• Translocation-associated notch protein TAN-1

Background

The notch1b gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NOTCH1