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Notch1b Antibody (SJ205)

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Western Blot: Notch1b Antibody (SJ205) [NBP2-67420] - Western blot analysis of Notch1b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-R
Western Blot: Notch1b Antibody (SJ205) [NBP2-67420] - Western blot analysis of Notch1b on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. ...read more
Immunocytochemistry/ Immunofluorescence: Notch1b Antibody (SJ205) [NBP2-67420] - Staining Notch 1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Notch1b antibody. The section was pre-treated using heat mediated ...read more
Flow Cytometry: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of Hela cells with Notch 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). ...read more
Western Blot: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of Notch 1 on HepG2 cell lysates using anti-Notch 1 antibody at 1/1,000 dilution.
Immunocytochemistry/ Immunofluorescence: Notch1b Antibody (SJ205) [NBP2-67420] - Staining Notch 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with ...read more
Immunocytochemistry/ Immunofluorescence: Notch1b Antibody (SJ205) [NBP2-67420] - Staining Notch 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised ...read more
Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded human lung tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded human spleen tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded mouse lung tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Analysis of paraffin-embedded mouse spleen tissue using anti-Notch 1 antibody. Counter stained with hematoxylin.
Immunohistochemistry-Paraffin: Notch1b Antibody (SJ205) [NBP2-67420] - Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Notch1b antibody. The section was pre-treated using heat mediated ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-P, IP
Clone
SJ205
Clonality
Monoclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

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Notch1b Antibody (SJ205) Summary

Additional Information
Recombinant Monoclonal Antibody.
Immunogen
Synthetic peptide within human Notch1b aa 2500-2540. (SwissProt: P46531 Human;SwissProt: Q01705 Mouse)
Localization
Cell membrane, Nucleus(intracellular domain).
Isotype
IgG
Clonality
Monoclonal
Host
Rabbit
Gene
NOTCH1
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
TBS (pH7.4), 1%BSA, 40%Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for Notch1b Antibody (SJ205)

  • EC 2.1.2.11
  • EC 3.4.21.68
  • hN1
  • Notch (Drosophila) homolog 1 (translocation-associated)
  • notch 1Notch homolog 1, translocation-associated (Drosophila)
  • Notch homolog 1, translocation-associated
  • TAN1neurogenic locus notch homolog protein 1
  • Translocation-associated notch protein TAN-1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Notch1b Antibody (SJ205)
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Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP

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Bioinformatics

Gene Symbol NOTCH1